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Current Nutrition & Food Science

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ISSN (Print): 1573-4013
ISSN (Online): 2212-3881

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Research Article

Microbiological Quality of Unpeeled Fruits Sold in the Local Market of Tangail District, Bangladesh, and Assessment of Health Impacts

Author(s): Md. Rayhan Ali, Safaiatul Islam, Md. Omar Faruque, Md. Firoz Ali, Fausiya Zaman Suha, Md. Tarikul Islam, Muhammad Wasim and Shahin Mahmud*

Volume 19, Issue 3, 2023

Published on: 09 September, 2022

Page: [329 - 337] Pages: 9

DOI: 10.2174/1573401318666220428120614

Price: $65

Abstract

Introduction: Fruits are good sources of vitamins, nutrients, minerals, micronutrients and fiber. However, fruits are usually eaten uncooked, which is hazardous for health as fruits are highly susceptible to microbial contamination during harvesting or postharvest processing.

Methods: Nine types of fruit that we usually eat without peeling were collected and tested them by using different standard microbiological methods. Biochemical and antibiogram tests were performed to confirm bacterial isolates and antibiotic-resistant patterns. An anthropometric assessment was also performed to evaluate whether common local fruits caused any prevalent digestive disorders.

Results: The highest Total Viable Count (TVC) was found in guava (1.95×105 CFU/g), whereas the highest Total Coliform Count (TCC) was identified in grapes (3.39×104 CFU/g). The mean value of TVC of all fruits was 7.112×103 CFU/g, and TCC was 6.02×102 CFU/g, respectively. Escherichia coli, Salmonella spp., Shigella spp., and Vibrio spp. were confirmed by biochemical tests. Antibiogram study shows that these bacterial isolates were resistant to commercially available antibiotics. E. coli isolates were resistant to Penicillin (73.3%); Salmonella spp. isolates were resistant to Penicillin (80%) and Amoxicillin (80%). Vibrio spp. isolates were resistant to Penicillin (66.67%), Amoxicillin, and Erythromycin (60%). The anthropometric assessment showed that acidity occurs more frequently than abdominal pain and digestive disorder among the correspondents. In the case of male and female respondents, black plum and apple were mostly responsible for gastric acidity, and the frequencies were 41% to 45%, respectively.

Conclusion: Fruit is a natural source of vitamins and minerals which are essential for our health; at the same time, steps must be taken to ensure that they are free from pathogenic microbes. Therefore, public awareness of proper hygiene and good health is necessary.

Keywords: Total Viable Count (TVC), Total Coliform Count (TCC), Colony Forming Unit (CFU), Anthropometric, Antibiogram, Antibiotic-resistant.

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[1]
Kalia A, Gupta RP. Fruit microbiology. Handb Fruits Fruit Process 2006; pp. 3-28.
[2]
Kaplan JE, Feldman R, Campbell DS, Lookabaugh C, Gary GW. The frequency of a Norwalk-like pattern of illness in outbreaks of acute gastroenteritis. Am J Public Health 1982; 72(12): 1329-32.
[http://dx.doi.org/10.2105/AJPH.72.12.1329] [PMID: 6291414]
[3]
Oranusi S, Olorunfemi OJ. Microbiological safety evaluation of street vended ready-to-eat fruits sold in Ota, Ogun state, Nigeria. Int J Res Biol Sci 2011; 1: 27-32.
[4]
Nguyen‐the C, Carlin F, Nguyen-The C. Critical reviews in food science and nutrition the microbiology of minimally processed fresh fruits and vegetables the microbiology of minimally processed fresh fruits and vegetables. Crit Rev Food Sci Nutr 1994; 34(4): 371-401.
[http://dx.doi.org/10.1080/10408399409527668]
[5]
Dunn RA, Hall WN, Altamirano JV, Dietrich SE, Robinson-Dunn B, Johnson DR. Outbreak of Shigella flexneri linked to salad prepared at a central commissary in Michigan. Public Health Rep 1995; 110(5): 580-6.
[PMID: 7480612]
[6]
Ofor MO, Okorie VC, Ibeawuchi II, Ihejirika GO, Obilo OP, Dialoke SA. Microbial contaminants in fresh tomato wash water and food safety considerations in South-Eastern Nigeria. Life Sci J 2009; 1: 80-2.
[7]
Ray B, Bhunia A. Fundamental food microbiology. (5th ed.). 2013; p. 664.
[http://dx.doi.org/10.1201/b16078]
[8]
Meleko A. Assessment of the sanitary conditions of catering establishments and food safety knowledge and practices of food handlers in Addis Ababa university students’ cafeteria. Sci J Public Heal 2015; 3(5): 733-43.
[http://dx.doi.org/10.11648/j.sjph.20150305.30]
[9]
Swanson KMJ. Fruits and fruit products. In: Micro-organism in foods 8. Springer, Boston: MA. 2011; pp. 177-95.
[10]
Cordier JL, Gram L, Tompkin RB, Gorris LGM, Swanson KMJ. Fruits and fruit products. In: Micro-organisms foods 6. Springer 2005; pp. 326-59.
[11]
Chukwu COC, Chukwu ID, Onyimba IA, Umoh EG, Olarubofin F, Olabode AO. Microbiological quality of pre-cut fruits on sale in retail outlets in Nigeria. Afr J Agric Res 2010; 5(17): 2272-5.
[12]
Hannan A, Rehman R, Saleem S, Khan MU, Qamar MU, Azhar H. Microbiological analysis of ready-to-eat salads available at different outlets in Lahore, Pakistan. Int Food Res J 2014; 21(5): 1797-800.
[13]
Farthing M, Salam MA, Lindberg G, et al. WGO. Acute diarrhea in adults and children: A global perspective. J Clin Gastroenterol 2013; 47(1): 12-20.
[http://dx.doi.org/10.1097/MCG.0b013e31826df662] [PMID: 23222211]
[14]
Beuchat LR. Ecological factors influencing survival and growth of human pathogens on raw fruits and vegetables. Microbes Infect 2002; 4(4): 413-23.
[http://dx.doi.org/10.1016/S1286-4579(02)01555-1] [PMID: 11932192]
[15]
Al-Kharousi ZS, Guizani N, Al-Sadi AM, Al-Bulushi IM, Shaharoona B. Hiding in fresh fruits and vegetables: Opportunistic pathogens may cross geographical barriers. Int J Microbiol 2016; 2016: 4292417.
[http://dx.doi.org/10.1155/2016/4292417] [PMID: 26989419]
[16]
Mahfuza I, Arzina H. Microbial status of street vended fresh-cut fruits, salad vegetables and juices in Dhaka city of Bangladesh. Int Food Res J 2016; 23(5): 2258-64.
[17]
Herigstad B, Hamilton M, Heersink J. How to optimize the drop plate method for enumerating bacteria. J Microbiol Methods 2001; 44(2): 121-9.
[http://dx.doi.org/10.1016/S0167-7012(00)00241-4] [PMID: 11165341]
[18]
Bhandari N, Nepali D, Paudyal S. Assessment of bacterial load in broiler chicken meat from the retail meat shops in Chitwan, Nepal. Int J Infect Microbiol 2013; 2(3): 99-104.
[http://dx.doi.org/10.3126/ijim.v2i3.8671]
[19]
Alwall N. Factors affecting the reliability of screening tests for bacteriuria. II. Dip-slide: False positive results following postal transport and false negatives owing to incubation at room temperature. Acta Med Scand 1973; 193(6): 505-9.
[http://dx.doi.org/10.1111/j.0954-6820.1973.tb10617.x] [PMID: 4578834]
[20]
Iacumin L, Comi G. Microbial quality of raw and ready-to-eat mung bean sprouts produced in Italy. Food Microbiol 2019; 82: 371-7.
[http://dx.doi.org/10.1016/j.fm.2019.03.014] [PMID: 31027796]
[21]
Hossain MB, Mahbub NDB, Chowdhury MMK, Rahaman MM. Molecular characterization of enterobacter and Escherichia coli pathotypes prevalent in the popular street foods of Dhaka city and their multidrug resistance. Bangladesh J Microbiol 2019; 34(2): 67-72.
[http://dx.doi.org/10.3329/bjm.v34i2.39614]
[22]
Hudzicki J. Kirby-bauer disk diffusion susceptibility test protocol author information. Am Soc Microbiol 2012; 15: 55-63.
[23]
Vandepitte J, Verhaegen J, Engbaek K, et al. Basic laboratory procedures in clinical bacteriology. World Health Organization 2003.
[24]
Kaur A, Kalan C, Sahib F, Bhowate P. Bacteriological anaylsis of fruits and vegetables from local market of Chunni Kalan, Fatehgarh Sahib Punjab. Pharma Innov J 2017; 11: 245-50.
[25]
Al Mamun S, Feroz F. Complete microbiological analysis of citrus fruits and the effect of heat on microbial load & antimicrobial activity. Stamford J Microbiol 2017; 7(1): 28-32.
[http://dx.doi.org/10.3329/sjm.v7i1.40068]
[26]
Sarker MAR, Haque MM, Rifa RA, Ema FA, Islam MA, Khatun MM. Isolation and identification of bacteria from fresh guava (Psidium guajava) sold at local markets in Mymensingh and their antibiogram profile. Vet World 2018; 11(8): 1145-9.
[http://dx.doi.org/10.14202/vetworld.2018.1145-1149] [PMID: 30250376]
[27]
Thin layer chromatographic profiling and phytochemical screening of six medicinal plants in Bangladesh. Int J Biosci 2017; 11(1): 131-40.
[http://dx.doi.org/10.12692/ijb/11.1.131-140]
[28]
Mortuza T. Isolation and identification of microbes from various fruit juices made and sold for immediate consumption at home and in the market of Dhaka city 2016; 53-6.
[29]
Balkhair KS. Microbial contamination of vegetable crop and soil profile in arid regions under controlled application of domestic wastewater. Saudi J Biol Sci 2016; 23(1): S83-92.
[http://dx.doi.org/10.1016/j.sjbs.2015.10.029] [PMID: 26858571]
[30]
Nkere CK, Ibe NI, Iroegbu CU. Bacteriological quality of foods and water sold by vendors and in restaurants in Nsukka, Enugu state, Nigeria: A comparative study of three microbiological methods. J Health Popul Nutr 2011; 29(6): 560.
[http://dx.doi.org/10.3329/jhpn.v29i6.9892]
[31]
Alam MK, Siddique MP, Hossain MT, Rahman MA, Khan MSR. Microbiological quality of market fruits and their handling implication in relation to public health. Microbes Health 2012; 1(2): 76-80.
[http://dx.doi.org/10.3329/mh.v1i2.14095]
[32]
Kagnoff MF, Eckmann L. Epithelial cells as sensors for microbial infection. J Clin Invest 1997; 100(1): 6-10.
[http://dx.doi.org/10.1172/JCI119522] [PMID: 9202050]
[33]
Portillo M del C, Franquès J, Araque I, Reguant C, Bordons A. Bacterial diversity of Grenache and Carignan grape surface from different vineyards at Priorat wine region (Catalonia, Spain). Int J Food Microbiol 2016; 219: 56-63.
[http://dx.doi.org/10.1016/j.ijfoodmicro.2015.12.002] [PMID: 26736065]
[34]
Shim WB, Kim KY, Yoon YH, et al. Microbiological hazard analysis for strawberry farms at the harvest stage to establish Good Agricultural Practices (GAP) model based on principle of HACCP. Korean J Food Sci Technol 2013; 45(1): 104-10.
[http://dx.doi.org/10.9721/KJFST.2013.45.1.104]
[35]
Hamad SH. The microbial quality of processed date fruits collected from a factory in Al-Hofuf City, Kingdom of Saudi Arabia. Emir J Food Agric 2012; 24(2): 105-12.
[36]
Graça A, Santo D, Esteves E, Nunes C, Abadias M, Quintas C. Evaluation of microbial quality and yeast diversity in fresh-cut apple. Food Microbiol 2015; 51: 179-85.
[http://dx.doi.org/10.1016/j.fm.2015.06.003] [PMID: 26187843]
[37]
Islam M, Islam M, Fakhruzzaman M. Isolation and identification of Escherichia coli and Salmonella from poultry litter and feed. Int J Nat Soc Sci 2014; 1(1): 1-7.
[38]
Hale TL, Keusch GT. Shigella Med Microbiol. 4th. 1996.
[39]
Grimont F, Lejay-Collin M, Talukder KA, et al. Identification of a group of Shigella-like isolates as Shigella boydii 20. J Med Microbiol 2007; 56(Pt 6): 749-54.
[http://dx.doi.org/10.1099/jmm.0.46818-0] [PMID: 17510258]
[40]
D’Aoust JY, Kushner DJ. Vibrio psychroerythrus sp. n.: Classification of the psychrophilic marine bacterium, NRC 1004. J Bacteriol 1972; 111(2): 340-2.
[http://dx.doi.org/10.1128/jb.111.2.340-342.1972] [PMID: 5053463]
[41]
Akond MA, Hassan SMR, Alam S, Shirin M. Antibiotic resistance of Escherichia coli isolated from poultry and poultry environment of Bangladesh. Am J Environ Sci 2009; 5(1): 47-52.
[http://dx.doi.org/10.3844/ajes.2009.47.52]
[42]
Mukherjee M, Basu S, Mukherjee SK, Majumder M. Multidrug-resistance and extended spectrum beta-lactamase production in uropathogenic E. coli which were isolated from hospitalized patients in Kolkata, India. J Clin Diagn Res 2013; 7(3): 449-53.
[http://dx.doi.org/10.7860/JCDR/2013/4990.2796] [PMID: 23634394]
[43]
Ali MR, Faruque MO, Islam MT, et al. Appraisal of heavy metal presence and water quality having microbial load and associated human health risk: A study on tube-well water in Nalitabari township of Sherpur district, Bangladesh. Grassroots J Nat Resour 2021; 4(2): 48-64.
[http://dx.doi.org/10.33002/nr2581.6853.040204]
[44]
Lee YJ, Kim AR, Jung SC, Song SW, Kim JH. Antibiotic resistance pattern of E. coli and Salmonella spp. isolated from chicken feces. Korean J Vet Res 2005; 45: 75-83.
[45]
Hassan M, Ahaduzzaman M, Alam M, Bari M, Amin K, Faruq A. Antimicrobial resistance pattern against E. coli and Salmonella spp. in environmental effluents. Int J Nat Sci 2016; 5(2): 52-8.
[http://dx.doi.org/10.3329/ijns.v5i2.28612]
[46]
Bantawa K, Sah SN, Subba Limbu D, Subba P, Ghimire A. Antibiotic resistance patterns of Staphylococcus aureus, Escherichia coli, Salmonella, Shigella and Vibrio isolated from chicken, pork, buffalo and goat meat in eastern Nepal. BMC Res Notes 2019; 12(1): 766.
[http://dx.doi.org/10.1186/s13104-019-4798-7] [PMID: 31752992]
[47]
Muinde OK, Kuria E. Hygienic and sanitary practices of vendors of street foods in Nairobi, Kenya. Afr J Food Agric Nutr Dev 2005; 5(8): 01-14.
[http://dx.doi.org/10.18697/ajfand.8.1060]
[48]
Barro N, Bello AR, Itsiembou Y, et al. Street-vended foods improvement: Contamination mechanisms and application of food safety objective strategy: Critical review. Pak J Nutr 2007.
[http://dx.doi.org/10.3923/pjn.2007.1.10]
[49]
Nicolas B, Abdoul RB, Aly S, Amadou TOC, Jules IA, Alfred ST. Hygienic status assessment of dish washing waters, utensils, hands and pieces of money from street food processing sites in Ouagadougou (Burkina Faso). Afr J Biotechnol 2006; 5(11): 1107-12.
[http://dx.doi.org/10.4314/ajb.v5i11.42982]
[50]
Mensah P, Yeboah-Manu D, Owusu-Darko K, Ablordey A. Street foods in Accra, Ghana: How safe are they? Bull World Health Organ 2002; 80: 546-54.
[51]
Scallan E, Hoekstra RM, Angulo FJ, et al. Foodborne illness acquired in the United States-major pathogens. Emerg Infect Dis 2011; 17(1): 7-15.
[http://dx.doi.org/10.3201/eid1701.P11101] [PMID: 21192848]

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