Title:A Sensitive Chromatographic Method for Hyaluronate Quantification Applied to Analyze the Desorption Behavior on Contact Lenses
Volume: 16
Issue: 6
Author(s): Wan-Hsin Chang, Pei-Yi Liu, Yuan-Ting Jiang*Yuan-Hao Howard Hsu*
Affiliation:
- R&D Center, Yung Sheng Optical Co., Taichung,Taiwan
- Department of Chemistry, Tunghai University, Taichung,Taiwan
Keywords:
Sodium hyaluronate, liquid chromatography, contact lenses, methafilcon, polymacon, silicone hydrogel.
Abstract:
Background: Sodium hyaluronate (NaHA) is generally supplemented in products related to
contact lenses for increasing comfort during wearing. The quantity of sodium hyaluronate and the material
of lenses affect the retention of sodium hyaluronate on the contact lenses.
Methods: We developed a convenient and sensitive but unconventional chromatographic method to
quantify sodium hyaluronate and analyze its release behavior from contact lenses. The reverse-phase
chromatography eluted sodium hyaluronate with high molecular masses in the shortest time and could
separate salt and small compounds from sodium hyaluronate.
Results: This method could accurately quantify sodium hyaluronate with diverse molecular sizes. Because
sodium hyaluronate was eluted in a narrow time frame, sensitivity was significantly enhanced,
and the limit of detection of this method was 0.45 μg/mL. According to this quantitation method, the
attached quantity of sodium hyaluronate is related to the water content of the material. Furthermore, a
material test indicated that the release efficiency of sodium hyaluronate depends on the material of
lenses. Nonionic Polymacon had a longer half-life in the sodium hyaluronate release curve than negative
Methafilcon A and silicone hydrogel.
Conclusion: This hyaluronate quantification method is a fast, sensitive and accurate method, making it
suitable for the in vitro hyaluronate research without further derivatization.
