Title:Protective Effect of Selenium-L-methionine on Radiation-induced Acute Pneumonitis and Lung Fibrosis in Rat
Volume: 14
Issue: 2
Author(s): Peyman Amini, Sedighe Kolivand, Hana Saffar, Saeed Rezapoor, Elahe Motevaseli*, Masoud Najafi*, Farzad Nouruzi, Dheyauldeen Shabeeb and Ahmed Eleojo Musa
Affiliation:
- Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran,Iran
- Radiology and Nuclear Medicine Department, School of Paramedical Sciences, Kermanshah University of Medical Sciences, Kermanshah,Iran
Keywords:
Selenium-L-methionine, lung, radiation, IL-4. IL-13, radiotherapy, pneumonitis, interleukin.
Abstract:
Background: In this study, we aimed to detect the changes in the level of interleukin
(IL)-4 and IL-13 cytokines and their downstream genes including interleukin-13 receptor subunit
alpha-2 (IL13Ra2), interleukin-4 receptor subunit alpha-1 (IL4Ra1), dual oxidase 1 (DUOX1) and
dual oxidase 2 (DUOX2). The protective effects of Selenium-L-methionine on radiation-induced
histopathological damages and changes in the level of these cytokines and genes were detected.
Methods: Four groups of 20 rats (5 rats in each) namely, control; Selenium-L-methionine, radiation
and radiation plus Selenium-L-methionine were used in this study. 4 mg/kg of Selenium-Lmethionine
was administered 1 day before irradiation and five consecutive days after irradiation.
Irradiation was done using a dose of 15 Gy 60Co gamma rays at 109 cGy/min. All rats were sacrificed
10 weeks after irradiation for detecting changes in IL-4 and IL-13 cytokines, the expressions
of IL13Ra2, IL4Ra1, Duox1 and Duox2 and histopathological changes.
Results: The level of IL-4 but not IL-13 increased after irradiation. This was associated with increased
expression of IL4Ra1, Duox1 and Duox2, in addition to changes in morphological properties.
Selenium-L-methionine could attenuate all injury markers following lung irradiation.
Conclusion: Selenium-L-methionine can protect lung tissues against toxic effects of ionizing radiation.
It is possible that the modulation of immune responses and redox interactions are involved in
the radioprotective effect of this agent.
