Title:How Moringa oleifera Supplementation Affects T-cell Subsets and Circulating Angiogenic, Myeloid, and Endothelial Cells in Mice with Alloxan-induced Diabetes
Volume: 18
Issue: 1
Author(s): Muobarak J. Tuorkey*, Abdel-Aziz A. Zidan and Enas A. Abdul Baki
Affiliation:
- Zoology Department, Faculty of Science, Damanhour University, Damanhour,Egypt
Keywords:
CD8+CD4+ T cells, lipid peroxidation, circulating angiogenic cells, immune modulation,
Moringa leaf, diabetes.
Abstract: Background and Aims: To our knowledge, no study has investigated neither
how Moringa leaf extract modulates the immune system in normal nor diabetic subjects.
Materials and Methods: Diabetes was induced by a single intraperitoneal dose of alloxan
(150 mg/kg). A dose of 100 mg/kg of MO extract was orally administered to diabetic
treated mice. The profile of glucose and insulin was evaluated. The activity of superoxide
dismutase, catalase, glutathione peroxidase, and glutathione enzymes was determined.
Liver function was monitored by measuring levels of alkaline phosphatase, glutamine pyruvate
transaminase, and glutamic oxaloacetic transaminase. The percentage of single-or
double-positive cells of each of CD8+/CD4+, CD117+/Sca-1+, Sca-1+/CD34+,
CD11b+/Ly6-G+, CD11c+/CD11b+, and CD34+/CD117+ was investigated by flow cytometry
in the peripheral blood.
Results: MO promotes the activity of both CD4+ and CD8+ T cells in diabetic treated
mice that may occur through the Sca-1+CD117+ stem cell factors. Administration of MO
leaf extract enhanced the percentage of the endothelial progenitors (CD34+CD117+) and
mature endothelial cells (CD34+CD117-). Moringa also increased the percentage of
blood-derived circulating angiogenic cells (Sca-1+/CD34+).
Discussion and Conclusions: This study shed light for the first time on how MO affects
different immune cells; the knowledge gained may help to overcome diabetes and its
complications.
