Title:Evaluation of Selected Parameters of Lipid Peroxidation and Paraoxonase Activity in Blood of Patients with Joint Osteoarthritis
Volume: 25
Issue: 9
Author(s): Dorota M. Olszewska-Slonina*, Stanisław Jung, Krzysztof J. Olszewski, Anna Cwynar and Gerard Drewa
Affiliation:
- Department of Pathobiochemistry and Clinical Chemistry, Collegium Medicum in Bydgoszcz, Nicolaus Copernicus University in Torun, Sklodowska-Curie 9, 85-094 Bydgoszcz,Poland
Keywords:
Aryldialkylphosphatase (paraoxonase), lipid peroxidation, malondialdehyde, osteoarthritis, oxidative stress,
Synovial fluid.
Abstract: Background: Oxidative stress constitutes an important risk factor for tissue/cell damage
and is involved in the occurrence and progression of Osteoarthritis (OA).
Objective: The aim of this study was to evaluate the concentration of Malondialdehyde (MDA) in
Synovial Fluid (SF) and blood as well as the activity of serum and SF paraoxonase (PON1) of patients
with OA. Our study aimed to assess local and systemic responses to oxidative stress.
Methods: The study included 43 subjects with OA qualified to total joint replacement. The control
group consisted of 58 subjects with no musculoskeletal disorders. The malondialdehyde level were
measured in venous blood plasma, erythrocytes (MDAe) and Synovial Fluid (SF) while PON1 activities
were estimated in serum and SF.
Results: No differences in MDA concentrations in erythrocytes and blood plasma between healthy
subjects and OA patients were observed. The significant higher level of MDA in SF compared with
blood serum was seen in both genders (in woman about 46.2%, in men - 75.0%) and in both assessed
joints (in hip about 53.8%, in knee - 115.9%). Total protein concentration and PON1 activity
in serum were significantly higher in OA patients (about 14.6% and 87.9% respectively). The systemic
response to osteoarthritis (serum PON1 activity) was higher about 33.6% in men than women
and not dependent on affected joint.
Conclusion: The changes observed in osteoarthritic SF show that the process of antioxidant enzymatic
defence is intensified locally, not systemically. The lipid hydroperoxides elimination may
lead to restore joints homeostasis.
