Title:Transplantation of Adipose Tissue-Derived Stem Cells into Brain Through Cerebrospinal Fluid in Rat Models: Protocol Development and Initial Outcome Data
Volume: 14
Issue: 2
Author(s): Naser Amini, Nasim Vousooghi, Akram Alizade, Sara Ramezani, Mohammad T. Joghataei*, Peiman Brouki Milan, Soraya Mehrabi, Shima Ababzadeh, Farshid Sefat and Masoud Mozafari*
Affiliation:
- Cellular and Molecular Research center, Iran University of Medical Sciences, Tehran,Iran
- Cellular and Molecular Research center, Iran University of Medical Sciences, Tehran,Iran
Keywords:
Nervous system, transplantation, stem cell, cerebrospinal fluid, brain tissue, adipose tissue.
Abstract:
Background: Cell therapy is an important strategy for the treatment of incurable diseases
including those that occur in the Central Nervous System (CNS). Among different strategies, the
method of delivering or transplantation of cells into the brain has shown significant effects on regeneration.
In this study, a new protocol has been developed for the transplantation of adipose tissuederived
stem cells into the brain through Cerebrospinal Fluid (CSF) in rat models.
Methods: For this purpose, a wide range of ages (7-30 days old) of male neonates of Wistar rats was
used. Moreover, human adipose tissue was obtained from a superficial layer of abdomen through liposuction
surgery. The size of the inserted part of needle to access middle cranial fossa and subarachnoid
space in animals with an average weight of 10-80 g was determined. In addition, to confirm the entrance
of needle into the subarachnoid space, CSF was aspirated slowly and then injection was done
within two minutes.
Results: The findings showed the presence of transplanted human Adipose-Derived Stem Cells
(hADSC) in the cerebellum and basal ganglia following three days and also after two months that confirmed
the entrance of transplanted cells into the cerebrospinal fluid and migration of them into the
brain tissue. All the animals survived after the transplantation process, with the lowest side effects
compared to the available conventional methods.
Conclusion: It can be concluded that the cells could be efficiently transplanted into CSF through subarachnoid
space by injection via superior orbital fissure with a minimally invasive technique.
