Title:Methodological Aspects of Phytosterol Measurements in Biological Samples
Volume: 26
Issue: 37
关键词:
金刚甾醇，菜油甾醇，菜油甾醇，胆固醇，气液色谱法，谷甾醇，谷甾烷醇，豆甾醇。
摘要: Phytosterol measurement has gained a lot of interest during the last two decades
after foods and supplements with added 4-desmethyl phytosterols were recognized and used
as effective and safe non-pharmacologic hypocholesterolemic agents, and also after the
mechanisms of intestinal absorption and hepatic excretion of sterols were unraveled. In addition,
the wide use of serum phytosterols as biomarkers of cholesterol absorption has increased
the interest in their measurement. In this review, the basic methods are discussed without going
into details of the practical operations. The analysis includes first lipid extraction and
saponification from various biologic matrices such as serum/plasma, feces, or tissues, after
which the individual sterols are separated by adsorption chromatography (gas-liquid or liquid
or high performance liquid chromatography) based on the polarity of the various sterols. We
also deal with some specific aspects of phytosterol measurements in biological samples such
as the need of harmonization of their analysis in biological samples, the discrepancies in the
results of sitosterol and campesterol concentrations between different studies, and what is
known about their biological day-to-day fluctuation. Phytosterols have a remarkable role in
human health, so that their complicated and time consuming measurements call attention to
routine ways of standardization between the sterol research laboratories.
