Title:LncRNA MALAT1 is Neuroprotective in a Rat Model of Spinal Cord Ischemia-Reperfusion Injury Through miR-204 Regulation
Volume: 15
Issue: 3
Author(s): Yong Qiao, Changliang Peng, Ji Li, Dongjin Wu and Xiuwen Wang*
Affiliation:
- Department of Spinal Surgery, The Second Hospital of Shandong University, Ji`nan,China
Keywords:
MALAT-1, miR-204, spinal cord, ischemia/reperfusion injury, oxygen, glucose deprivation.
Abstract: Background: This study was to investigate the neuroprotective effect of long noncoding
RNAs Metastasis associated lung adenocarcinoma transcript-1 (lncRNA MALAT-1) in
Spinal Cord Ischemic/Reperfusion Injury (SCIRI).
Methods: Quantitative real-time PCR (RT-qPCR) was used to examine the expressions of
MALAT1, miR-204 and Bcl-2, while western blot was performed to examine Bcl-2. Besides,
apoptosis was evaluated by the percentage of cell viability and apoptotic cells. Neurological
evaluation was performed by measuring hindlimb locomotor function.
Results: The expression of MALAT1 and Bcl-2 was decreased, while miRNA-204 (miR-204) was
up-regulated in rats SCIRI model and neurocyte lines under hypoxic conditions. Oxygen, Glucose
Deprivation (OGD) promoted apoptosis of neurocytes, downregulated MALAT1 and Bcl-2 and
elevated miR-204 expression, however, overexpression of MALAT1 notably reversed this trend.
Nevertheless, knockdown of MALAT1 increased cell apoptosis, decreased MALAT1 and Bcl-2
but upregulated miR-204. MALAT1 overexpression-induced anti-apoptosis and knockdowninduced
pro-apoptosis were obviously reversed by synchronously overexpression and knockdown
of miR-204, respectively. MALAT1-treated SCIRI rats exhibited lower Motor Deficit Index
(MDI) scores, higher levels of MALAT1 and Bcl-2 expression and lower miR-204 expression.
Conclusion: Our data suggested that MALAT1 exerted neuroprotective effect in a rat model of
SCIRI by regulating miR-204.
