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Current Pharmaceutical Analysis

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ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

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Research Article

Tissue Distribution of Engeletin in Mice by UPLC-MS/MS

Author(s): Weijian Ye, Chongliang Lin, Guanyang Lin, Ruijie Chen, Wei Sun, Shuanghu Wang, Xianqin Wang* and Yunfang Zhou*

Volume 15, Issue 6, 2019

Page: [604 - 611] Pages: 8

DOI: 10.2174/1573412914666180501114659

Price: $65

Abstract

Introduction: Engeletin is the main active component in the engelhardia leaf that promotes circulation and removes stasis, and has hypoglycemic, hypolipidemic, and anti-inflammatory actions. The aim of this study was to develop an ultra-performance liquid chromatography- tandem mass spectrometry method to detect engeletin in plasma and tissues and investigate its absorption, distribution, and mechanism in mice, which could provide very useful information for its pharmacological effect in vivo.

Materials and Methods: Twenty-five mice were intraperitoneally injected with 20 mg/kg engeletin, and five mice were sacrificed using 4% chloral hydrate 0.25, 0.5, 2, 4, and 6 h later. The tissues (brain, kidney, heart, liver, spleen, and lung) and blood were collected. Acetonitrile precipitation was applied to remove protein and further process the mouse plasma and tissue homogenate samples. Multiple reactions monitoring mode in negative mode was used to quantify the engeletin.

Results and Conclusion: Linearity of engeletin in plasma and tissues was good (R2 > 0.995), within the range of 2-2,000 ng/mL in plasma and 2-2,000 ng/g in tissues, and the lower limit of quantitation was 2 ng/mL in plasma and 2 ng/g in tissues. Inter-day precision of engeletin in plasma or tissues (brain, kidney, heart, liver, spleen, and lung) was < 14%, and intra-day precision was < 15%. After the mice were intraperitoneally injected with engeletin (20 mg/kg), the distribution in kidney and liver was the highest, followed by blood, spleen, lung, heart, and brain. Engeletin concentration in the brain was low, suggesting that engeletin can penetrate through the blood brain barrier, which could also help with engeletin investigations of the brain.

Keywords: Engeletin, UPLC-MS/MS, mouse, tissue, plasma, Engelhardia roxburghiana.

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[1]
Shi, H.; Liu, M.; Wang, R.; Gao, B.; Zhang, Z.; Niu, Y.; Yu, L.L. Separating four diastereomeric pairs of dihydroflavonol glycosides from Engelhardia roxburghiana using high performance counter-current chromatography. J. Chromatogr. A, 2015, 1383, 79-87.
[2]
Lin, W.Y.; Peng, C.F.; Tsai, I.L.; Chen, J.J.; Cheng, M.J.; Chen, I.S. Antitubercular constituents from the roots of Engelhardia roxburghiana. Planta Med., 2005, 71, 171-175.
[3]
Huang, H.; Cheng, Z.; Shi, H.; Xin, W.; Wang, T.T.; Yu, L.L. Isolation and characterization of two flavonoids, engeletin and astilbin, from the leaves of Engelhardia roxburghiana and their potential anti-inflammatory properties. J. Agric. Food Chem., 2011, 59, 4562-4569.
[4]
Wu, H.C.; Cheng, M.J.; Peng, C.F.; Yang, S.C.; Chang, H.S.; Lin, C.H.; Wang, C.J.; Chen, I.S. Secondary metabolites from the stems of Engelhardia roxburghiana and their antitubercular activities. Phytochemistry, 2012, 82, 118-127.
[5]
Wu, C.C.; Peng, C.F.; Tsai, I.L.; Abd El-Razek, M.H.; Huang, H.S.; Chen, I.S. Secondary metabolites from the roots of Engelhardia roxburghiana and their antitubercular activities. Phytochemistry, 2007, 68, 1338-1343.
[6]
Wu, H.; Zhao, G.; Jiang, K.; Li, C.; Qiu, C.; Deng, G. Engeletin alleviates lipopolysaccharide-induced endometritis in mice by inhibiting TLR4-mediated NF-kappaB activation. J. Agric. Food Chem., 2016, 64, 6171-6178.
[7]
Yang, W.; Sabi-Mouka, E.M.B.; Wang, L.; Shu, C.; Wang, Y.; Ding, J.; Ding, L. Determination of tranilast in bio-samples by LC-MS/MS: Application to a pharmacokinetic and brain tissue distribution study in rats. J. Pharm. Biomed. Anal., 2017, 147, 479-484.
[8]
Wang, Z.; You, L.; Chen, Y.; Hu, K.; Wang, Z.; Cheng, Y.; Yang, J.; Yang, Y.; Wang, G. Investigation of pharmacokinetics, tissue distribution and excretion of Schisandrin B in rats by HPLC-MS/MS. Biomed. Chromatogr., 2018, 32(2)
[http://dx.doi.org/10.1002/bmc.4069]
[9]
Wang, X.Q.; Wang, S.H.; Lin, F.Y.; Zhang, Q.W.; Chen, H.L.; Wang, X.C.; Wen, C.C.; Ma, J.S.; Hu, L.F. Pharmacokinetics and tissue distribution model of cabozantinib in rat determined by UPLC-MS/MS. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2015, 983, 125-131.
[10]
Ye, W.; Chen, R.; Sun, W.; Huang, C.; Lin, X.; Dong, Y.; Wen, C.; Wang, X. Determination and pharmacokinetics of engeletin in rat plasma by ultra-high performance liquid chromatography with tandem mass spectrometry. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2017, 1060, 144-149.
[11]
Mordenti, J.; Cuthbertson, R.A.; Ferrara, N.; Thomsen, K.; Berleau, L.; Licko, V.; Allen, P.C.; Valverde, C.R.; Meng, Y.G.; Fei, D.T.; Fourre, K.M.; Ryan, A.M. Comparisons of the intraocular tissue distribution, pharmacokinetics, and safety of 125I-labeled full-length and Fab antibodies in rhesus monkeys following intravitreal administration. Toxicol. Pathol., 1999, 27, 536-544.
[12]
Gutierrez-Puente, Y.; Tari, A.M.; Stephens, C.; Rosenblum, M.; Guerra, R.T.; Lopez-Berestein, G. Safety, pharmacokinetics, and tissue distribution of liposomal P-ethoxy antisense oligonucleotides targeted to Bcl-2. J. Pharmacol. Exp. Ther., 1999, 291, 865-869.
[13]
Xu, W.; Zhang, Y.; Zhou, C.; Tai, Y.; Zhang, X.; Liu, J.; Sha, M.; Huang, M.; Zhu, Y.; Peng, J.; Lu, J.J. Simultaneous quantification six active compounds in rat plasma by UPLC-MS/MS and its application to a pharmacokinetic study of Pien-Tze-Huang. J. Chromatogr. B Analyt. Technol. Biomed. Life Sci., 2017, 1061-1062, 314-321.
[14]
Wang, Z.; Zhao, Q.; Li, L.; Hu, P.; Dong, K.; Chen, S.; Jiang, J. Development and validation of a rapid and sensitive UPLC-MS/MS method for quantification of kukoamine B in human plasma: Application to a clinical pharmacokinetic study. J. Pharm. Biomed. Anal., 2017, 132, 1-6.
[15]
Wang, W.; Zheng, X.; Wang, H.; Wang, L.; Jiang, J.; Hu, P. Development of an UPLC-MS/MS method for quantification of Avitinib (AC0010) and its five metabolites in human cerebrospinal fluid: Application to a study of the blood-brain barrier penetration rate of non-small cell lung cancer patients. J. Pharm. Biomed. Anal., 2017, 139, 205-214.
[16]
Szeitz, A.; Manji, J.; Riggs, K.W.; Thamboo, A.; Javer, A.R. Validated assay for the simultaneous determination of cortisol and budesonide in human plasma using ultra high performance liquid chromatography-tandem mass spectrometry. J. Pharm. Biomed. Anal., 2014, 90, 198-206.
[17]
Zhang, Q.; Wen, C.; Xiang, Z.; Ma, J.; Wang, X. Determination of CUDC-101 in rat plasma by liquid chromatography mass spectrometry and its application to a pharmacokinetic study. J. Pharm. Biomed. Anal., 2014, 90, 134-138.
[18]
Lin, G.Y.; Wu, H.Y.; Wang, Z.B.; Zhang, H.Y.; Chen, L.G.; Wang, X.Q.; Hu, L.F.; Ma, J.S. Determination of sulpiride in rabbit plasma by LC-ESI-MS and its application to a pharmacokinetic study. Lat. Am. J. Pharm., 2011, 30, 1775-1779.
[19]
Lin, G.Y.; Hu, L.F.; Yang, X.Z.; Pan, X.J.; Wang, X.Q. Determination of gemcitabine in rabbit plasma by lc-esi-ms using an allure PFP propyl column. Lat. Am. J. Pharm., 2011, 30, 571-575.
[20]
Guo, J.Y.; Xu, Q.Q.; Tong, S.H.; Wang, S.H.; Zhang, Q.W.; Sun, F.; Wang, X.Q. The effect of transmetil on pharmacokinetics of MS-275 in rats. Lat. Am. J. Pharm., 2014, 33, 1567-1570.
[21]
Gullick, D.R.; Mott, K.B.; Bartlett, M.G. Chromatographic methods for the bioanalysis of pyrethroid pesticides. Biomed. Chromatogr., 2016, 30, 772-789.
[22]
Yang, X.K.; Ma, Y.J.; Li, N.; Cai, H.J.; Bartlett, M.G. Development of a method for the determination of Acyl-CoA compounds by liquid chromatography mass spectrometry to probe the metabolism of fatty acids. Anal. Chem., 2017, 89, 813-821.
[23]
Ding, C.; Dong, W.; Yang, F.F.; Sun, W.; Chen, X.L.; Ma, J.S.; Wang, X.Q.; Hu, L.F. Determination of ibudilast in rabbit plasma by liquid chromatography-mass spectrometry and its application. Lat. Am. J. Pharm., 2011, 30, 2065-2069.
[24]
Cai, J.Z.; Huang, Z.Z.; Chen, X.L.; Xu, R.A.; He, H.Z.; Lin, C.L.; Lin, G.Y.; Wang, X.Q. Simultaneous determination of three bioactive compounds in traditional chinese medicine patent prescription zuojin pill by HPLC. Lat. Am. J. Pharm., 2012, 31, 388-393.
[25]
Wang, S.H.; Wu, H.Y.; Huang, X.L.; Geng, P.W.; Wen, C.C.; Ma, J.S.; Zhou, Y.F.; Wang, X.Q. Determination of N-methylcytisine in rat plasma by UPLC-MS/MS and its application to pharmacokinetic study. J. Chromatogr. B Ana. Technol. Biomed. Life Sci., 2015, 990, 118-124.
[26]
Ma, J.S.; Wang, S.H.; Huang, X.L.; Geng, P.W.; Wen, C.C.; Zhou, Y.F.; Yu, L.S.; Wang, X.Q. Validated UPLC-MS/MS method for determination of hordenine in rat plasma and its application to pharmacokinetic study. J. Pharmaceut. Biomed. Anal., 2015, 111, 131-137.
[27]
Wang, W.; Liu, J.; Zhao, X.; Peng, Y.; Wang, N.; Lee, D.Y.; Dai, R. Pharmacokinetics, tissue distribution, and excretion studies of l-isocorypalmine using ultra high performance liquid chromatography with tandem mass spectrometry. J. Sep. Sci., 2017, 40, 1040-1048.
[28]
He, M.; Ouyang, H.; He, M.; Tan, T.; Li, J.; Zhang, X.; Jia, J.; Feng, Y.; Yang, S. Application of a liquid chromatography-tandem mass spectrometry method to the pharmacokinetics, tissue distribution and excretion in the study of anemoside B4, a novel antiviral agent candidate, in rats. Biomed. Chromatogr., 2017, 31
[http://dx.doi.org/10.1002/bmc.3914]
[29]
Bai, Y.; Zhang, Q.; Wang, B.; Zhang, M.; Xu, Y.; Li, S.; Zhao, Y.; Yu, Z. Plasma pharmacokinetics, bioavailability, and tissue distribution of four C-Glycosyl flavones from mung bean (Vigna radiata L.) seed extracts in rat by ultrahigh-performance liquid chromatography-tandem mass spectrometry. J. Agric. Food Chem., 2017, 65, 5570-5580.

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