Title:Apicidin Inhibited Proliferation and Invasion and Induced Apoptosis via Mitochondrial Pathway in Non-small Cell Lung Cancer GLC-82 Cells
Volume: 17
Issue: 10
Author(s): Jianye Zhang, Zhenzhu Lai, Wenjing Huang, Huiping Ling, Minting Lin, Sili Tang, Yun Liu and Yiwen Tao*
Affiliation:
- Department of Medicinal Chemistry, School of Pharmaceutical Sciences, Guangzhou Medical University, Guangzhou 511436,China
Keywords:
Apicidin, Fusarium sp, histone deacetylase, mitochondrial pathway, apoptosis, proliferation, invasion.
Abstract: Background: Apicidin, as an inhibitor of histone deacetylase, showed a wide range of antiproliferative
activity against various cancer cell lines. Apicidin has also been reported to induce apotosis via Fas/Fas
ligand. Yet few studies have been focused on mitochondrial pathway for its anti-tumor activity.
Objective: In this study, we evaluated its involved mitochondrial mechanism against non-small cell lung cancer
GLC-82 cells.
Method: Apicidin was isolated from the mangrove endophtic fungi Fusarium sp. by solvent extraction and
column chromatography. Its structure was elucidated by MS and NMR spectroscopic data, and comparison of
those data with published data. Furthermore, anti-tumor activity and mitochondrial pathway of apicidin against
GLC-82 cells were studied.
Results: Apicidin was obtained from secondary metabolites of Fusarium sp., and it showed potent inhibitory
activity against GLC-82 cells with the IC50 value of 6.94 ± 0.27 µM. Furthermore, apicidin suppressed proliferation
and invasion, and induced apoptosis via mitochondrial pathway in GLC-82 cells, including loss of ΔΨm,
release of cytochrome c from mitochondria, activation of caspase-9 and -3, and cleavage of poly-ADP-ribose
polymerase.
Conclusion: Apicidin, an inhibitor of histone deacetylase obtained from the mangrove endophytic fungi
Fusarium sp., not only inhibited proliferation and invasion of GLC-82 cells, but also induced apoptosis via the
mitochondrial pathway.
