Title:Ferulenol, a Sesquiterpene Coumarin, Induce Apoptosis via Mitochondrial Dysregulation in Lung Cancer Induced by Benzo[a]pyrene: Involvement of Bcl2 Protein
Volume: 17
Issue: 10
Author(s): Nesrine Lariche, Mesbah Lahouel*, Lamia Benguedouar and Ammar Zellagui
Affiliation:
- Laboratory of Molecular Toxicology, Faculty of Sciences, University Mohammed Seddik Benyahia of Jijel, 18000,Algeria
Keywords:
Benzo[a]pyrene, ferulenol, lung cancer, bcl2 protein, chemotherapeutic, pro-oxidant.
Abstract: Background: Ferulenol, a sesquiterpene coumarin, was extracted from “Ferula vesceritensis” and
possesses pro-oxidative and anticancer effects in different types of cancer.
Objective: The objective of this study is to determine whether ferulenol has an anticancer effect by regulation the
Bcl2 protein expression in lung cancer induced by benzo[a]pyrene in Wistar rats.
Methods: Rats in group 1 have received, intraperitoneally, olive oil and considered as controls, animals of group
2 were treated with 100 mg/kg of benzo[a]pyrene intraperitoneally in order to induce lung cancer for 24 weeks,
the 3rd groups of rats received the ferulenol 50 mg/kg intraperitoneally after 24 weeks of administration of
benzo[a]pyrene and the last group, the rats were treated with ferulenol alone 50 mg/kg.
Results: Treatment with ferulenol significantly increased the rate of lipid peroxidation and decrease enzymatic
(CAT and GST) and non-enzymatic (GSH) anti-oxidants in benzo[a]pyrene induced lung cancer. Anticancer
efficacy of ferulenol was evaluated by down-regulation of Bcl2 protein and up-regulation of Bax protein,
Therefore, ferulenol can alter the functionality of lung mitochondria by increasing the production of superoxide
anion and mitochondrial swelling.
Conclusion: Together, our results depict that ferulenol can be used as pro-oxidant and chemotherapeutic agent
against lung cancer.
