Title:Phosphoproteomics: Protein Phosphorylation in Regulation of Seed Germination and Plant Growth
Volume: 19
Issue: 4
Author(s): Xiaojian Yin, Xin Wang and Setsuko Komatsu*
Affiliation:
- Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba 305-8572,Japan
Keywords:
Phosphorylation, proteomics, plant, germination, growth, phytohormones.
Abstract: Seed germination and plant growth are key stages in the plant life cycle and they are affected
by abiotic stresses. Protein phosphorylation is commonly involved in key regulatory process, which mediates
plant growth/development and stress responses. In plant, phosphoproteins play roles in stress responses
and provide basic backbone in complex signaling networks. The mechanism of plant responses
to abiotic stresses has been uncovered through study of protein phosphorylation. Recently, new methods
for enrichment of phosphopeptides and phosphoproteins have been developed. Phosphoproteomic techniques
are widely applied to studies of seed germination and plant growth. The signaling networks of
phytohormones have been explored using the phosphoproteomic approach. In this review, the enrichment
methods of phosphopeptides and phosphoproteins are summarized for phosphoproteomics. The roles of
protein kinases and phosphatases in plant are described. The significant achievements of phosphoproteomics
in the areas of seed germination and plant growth under abiotic stresses are reviewed. Applications
of phosphoproteomics are valuable resources to uncover functional proteins regulating plant growth
and stress responses.
