Title:Probiotic Properties of Lactobacillus fermentum Strains Isolated from Human Oral Samples and Description of their Antibacterial Activity
Volume: 18
Issue: 2
Author(s): Virginia Fuochi, Giovanni Li Volti and Pio M. Furneri
Affiliation:
Keywords:
Antibacterial activity, sensitivity test, resistance, cytotoxicity, Lactobacillus, probiotics.
Abstract: Background: Gram positive bacteria produce peptides, defined bacteriocins which exhibit
good antibacterial activity.
Objective: We evaluated the ability of L. fermentum to produce bacteriocins having therefore, good probiotic
features and finally, be safe towards microglial cells.
Method: Eight wild strains, identified using molecular techniques, were investigated for the evaluation
of resistance to bile salts, low pH, H2O2 production, biofilm formation, antibacterial activity and safety
on microglia cells (BV2).
Results: The determination of the susceptibility/resistance profile showed that the strains are sensitive to
the antibiotics tested. All strains showed a good tolerability to extremely low pH as well as resisting in
presence of bile salts. In addition, the strains showed excellent activity against pathogens and one of
them (LAC 42) showed activity also against Pseudomonas aeruginosa and Klebsiella pneumoniae. Finally,
LAC 42 and its active compound did not change microglia cell viability following 24h exposure.
Our data on this antibacterial molecule suggest that it is a compound with low molecular weight and
with highly hydrophilic component.
Conclusion: These results describe the characteristics of Lactobacillus strains and provide evidences for
their possible use as new potential probiotic. In addition, other studies are now warranted to exploit the
antibacterial activity of the supernatant LAC 42 and for its complete chemical characterization.
