Title:In vitro Vasorelaxant Effect of Artemisia herba alba Asso. in Spontaneously Hypertensive Rats
Volume: 14
Issue: 3
Author(s): Zeggwagh Naoufel, Morad Hebi, Mohammed Ajebli, Jean B. Michel and Mohamed Eddouks
Affiliation:
Keywords:
Aortic rings, Artemisia herba alba, endothelium vasorelaxant factors, ethnophamacology, hypertension, nitric oxide
synthesis.
Abstract: Background: The present study aims to evaluate the in vitro vasorelaxant effect of Artemisia
herba alba (Ah) aqueous extract.
Method: Aortic rings were isolated from spontaneously hypertensive rats and incubated in aqueous Ah
extract at the following doses: 3, 5, 10 and 20 mg/ml. Incubation of aqueous Ah extract for 30 minutes
produced a significant shift of the dose response curve to Norepinephrine (NE) (10
-8 to 10
-5 M)
(p<0.001).
Results: Endothelium ablation significantly reduced the vasorelaxant effect of aqueous Ah extract
(p<0.001). In addition, inhibition of vascular nitric oxide by N
ω-Nitro-L-Arginine Methyl (LNAME)
produced a significant reduction in the vasorelaxant effect of aqueous Ah extract (p<0.001).
Conclusion: We conclude that aqueous Ah extract at a dose of 20 mg/ml possess an in vitro vasorelaxant
effect which seems to be dependent on the endothelium vasorelaxant factors involving nitric oxide
synthesis.
