Title:Phenolic Profile and Antioxidant Activity of Centaurea choulettiana Pomel (Asteraceae) Extracts
Volume: 19
Issue: 10
Author(s): Djihane Azzouzi, Kenza Bioud, Ibrahim Demirtas, Fatih Gul, Djamel Sarri, Samir Benayache, Fadila Benayache and Ratiba Mekkiou
Affiliation:
Keywords:
Centaurea choulettiana, phenolic acids, HPLC-TOF/MS, antioxidant activity, total phenolics, total flavonoids.
Abstract: Aim and objective: This study aimed to quantify phenolic compounds in
ethyl acetate and n-butanol extract of Centaurea choulettiana Pomel (Asteraceae)
leaves and flowers; compare the antioxidant activity of their extracts, identification
and quantification of their phenolic acids.
Materials and methods: Both organs extracts of Centaurea choulettiana Pomel were
investigated and evaluated for their potential antioxidant properties using total
phenolics and flavonoids content, DPPH radical scavenging and lipid peroxidation
inhibition assays. HPLC-TOF/MS analyses were carried out to identify and quantify
some phenolic acids.
Results: The amounts of phenolic and flavonoid content were higher in ethyl acetate
extract of leaves (325.81 ± 0.038 mgGAE and 263.73 ± 0.004 mgQE /g of extract) respectively.
Besides, this extract exhibited the most powerful effect on the DPPH radical scavenging activity with
(96.54%), on lipid peroxydation inhibition (64.17%). Ethyl acetate extract of leaves and flowers were
found to contain almost the same phenolic compounds, with the leaves having the highest values.
Chlorogenic acid was detected in the n-butanol extract of flowers with the highest concentration 17.78
mg/kg plant.
Conclusion: The ethyl acetate extract of leaves of Centaurea choulettiana possesses strong
antioxidative properties in vitro. They are confirmed by high polyphenols and flavonoids content. The
HPLC-TOF/MS analysis reveals the presence of 4-hydroxybenzoic acid, gentisic acid, chlorogenic
acid, caffeic acid, vanillic acid, p-Coumaric acid, ferulic acid, salicylic acid and protocatechuic acid.
