Cell-Free Technologies for Proteomics and Protein Engineering

Title:Cell-Free Technologies for Proteomics and Protein Engineering

Volume: 23 Issue: 9

Author(s): Hiroyuki Ohashi and Etsuko Miyamoto-Sato

Affiliation:

Keywords: Cell-free, IVV (in vitro virus), mRNA display, PPI (protein-protein interaction), Proteomics, PURE system.

Abstract: Cell-free translation systems facilitate rapid production of specific proteins and are particularly suited as high-throughput methods for whole-genome protein synthesis. Moreover, these systems do not rely on living cells, thereby allowing the synthesis of unstable or cytotoxic proteins in vitro. In this review, we describe the principles and potential applications of cell-free protein translation systems and the future prospects of proteomics approaches using next-generation sequencing and cell-free expression technologies.

Cite this article as:

Ohashi Hiroyuki and Miyamoto-Sato Etsuko, Cell-Free Technologies for Proteomics and Protein Engineering, Protein & Peptide Letters 2016; 23 (9) . https://dx.doi.org/10.2174/0929866523666160613200304