Title:Critical Review Upon the Role and Potential of Fluorescence and Near-Infrared Imaging and Absorption Spectroscopy in Cancer Related Cells, Serum, Saliva, Urine and Tissue Analysis
Volume: 23
Issue: 27
Author(s): Christian W. Huck, Yukihiro Ozaki and Verena A. Huck-Pezzei
Affiliation:
Keywords:
Fluorescence, Near Infrared, Spectroscopy, Cells, Serum, Urine, Saliva, Tissue.
Abstract: During the last years, non-invasive or minimally invasive diagnostic
tools in cancer diagnostics have become more important. Many fluorescence
spectroscopic methodologies have been established for nearly all
different kinds of cancer. The reason therefore is its high sensitivity, low
amount of sample required, short testing time, and the suitability for in situ
testing. The potential influence factors for cancer diagnostics and the subsequent
suitability of the method to different applications are well described.
Near-Infrared spectroscopy (NIRS) is based on differences of endogenous
chromophores between cancer and normal tissues using either oxyhaemoglobin
or deoxy-haemoglobin, lipid or water bands, or a combination of two or more
of these diagnostic markers. These marker bands are known to provide the fundamental for
the diagnosis of several cancers and the spectroscopic setup can be applied for the analysis
of cells, urine and tissue. For the preparation of this review the literature published during
the last fifteen years has been taken into consideration. It will provide an overview on the
importance of the fluorescence and NIRS tools in cancer analysis giving hints about how
these techniques can play a crucial role in cancer diagnosis, treatment decisions and therapy.
The two techniques, fluorescence and near-infrared spectroscopy (NIRS) are faced to each
other and individual advantages and/or drawbacks are discussed. Finally, it will be taken
into consideration; how the synergistic combination of different approaches can give additional
information related to development and progression stages of cancer.
