Title:Meta-analysis Reveals No Association of DNMT3B -149 C>T Gene Polymorphism With Overall Cancer Risk
Volume: 17
Issue: 6
Author(s): Raju Kumar Mandal, Shafiul Haque, Mohd Wahid, Arshad Jawed, Naseem Akhter, Md. Ekhlaque Ahmed Khan, Aditya Kumar Panda, Mohammed Yahya Areeshi and Sajad Ahmad Dar
Affiliation:
Keywords:
DNA methyltransferase-3B, Polymorphism, Cancer, Meta-analysis, Asians, Caucasians.
Abstract: Background: DNA methyltransferase-3B (DNMT3B) plays a key role
in establishment and maintenance of genomic methylation patterns. Polymorphism
in promoter region -149 C>T (C46359T) of DNMT3B gene may alter DNMT3B
activity which leads to increased susceptibility to cancer. Inconsistent results regarding
this have been reported in a number of studies.
Objective: To carry out a meta-analysis of the studies reported to assess the precise
relationship between the DNMT3B -149 C>T polymorphism and the overall cancer
risk.
Method: PubMed (MEDLINE) web database was searched for the studies concerning
DNMT3B -149 C>T polymorphism and its association with cancer risk.
The pooled odds ratios (ORs) along with 95% confidence intervals (95% CIs) were calculated for all
the genetic models, from the selected case-control studies, by meta-analysis.
Results: Overall eighteen studies containing 5583 cancer cases and 7618 controls were analyzed. No
significant risk was observed overall for T allele carrier (T vs. C: p=0.303; OR=1.032, 95% CI=0.972-
1.097), homozygous (TT vs. CC: p=0.336; OR=1.063, 95% CI=0.939–1.204), heterozygous (CT vs.
CC: p=0.802; OR=1.022, 95% CI=0.860-1.216), dominant (TT vs. CC+CT: p=0.298; OR=1.101, 95%
CI=0.919-1.319) and recessive (TT+CT vs. CC: p=0.656; OR=1.021, 95% CI=0.931-1.121) genetic
models. Subgroup analysis of Asian and Caucasian populations also did not demonstrate any cancer
risk in all the genetic models studied.
Conclusion: Our meta-analysis proposes that the DNMT3B -149 C>T polymorphism may not be an
independent predisposing factor for the risk of cancer. However, larger sample size and expression
studies are required to confirm the observation.
