Title:In Vitro Anticancer Effects of Two New Potent Hydrazide Compounds on Leukemic Cells
Volume: 16
Issue: 12
Author(s): Shahrzad Tavakolfar, Elham Mousavi, Ali Almasirad, Amir Amanzadeh, Seyyed Mohamamd Atyabi, Parichehr Yaghamii, Somayeh Samiee-Sadr and Mona Salimi
Affiliation:
Keywords:
Leukemia, apoptosis, cancer, hydrazide.
Abstract: Background: Chronic myeloid leukemia (CML), also recognized as chronic
myelogenous leukemia, is initiated in some types of blood-forming cells of the bone marrow.
The therapeutic approach to CML is usually chemotherapy; however, severe side effects and
complications are major problems in the clinical research. Thus, recent efforts have focused on
the search for compounds affecting apoptosis in this type of cancer.
Objective: In this study, in vitro anticancer activity of two compounds (A and B) consisting of a
hydrazide backbone with nitro-thiophen and furan substituents was assessed against K562 cell
line displaying certain levels of sensitivity to pro-apoptotic compounds.
Methods: The anticancer activity was assessed using MTT assay, flowcytometry, annexin-V and
Western blot analysis.
Results: Compounds A and B were both active and revealed a remarkable in vitro cytotoxic
effect showing IC50 values of 0.09 and 0.07 μM, respectively, after 72 h of treatment. A significant increase in
annexin-V/PI staining, sub-G1 population and Bax/Bcl-2 ratio revealed the apoptotic cell death of compounds A- and
B-treated K562 cells.
Conclusion: The results presented here could be used as a first step for the development of powerful chemotherapeutic
agents to treat leukemia.
