Title:Effect of a Metalloantibiotic Produced by Pseudomonas aeruginosa on Klebsiella pneumoniae Carbapenemase (KPC)-producing K. pneumoniae
Volume: 17
Issue: 4
Author(s): Gilselena Kerbauy, Ana C.P. Vivan, Glenda C. Simões, Ane S. Simionato, Marsileni Pelisson, Eliana C. Vespero, Silvia F. Costa, Celia G.T. de J. Andrade, Daiane M. Barbieri, João C.P. Mello, Alexandre T. Morey, Lucy M. Yamauchi, Sueli F. Yamada-Ogatta, Admilton G. de Oliveira and Galdino Andrade
Affiliation:
Keywords:
Antibiotic activity, biofilm, fraction, control, natural product, KPC.
Abstract: Multidrug-resistant organisms (MDRO) are a great problem in hospitals, where thousands
of people are infected daily, with the occurrence of high mortality rates, especially in infections caused
by Klebsiella pneumoniae carbapenemase-producing K. pneumoniae (KPC-producing Kpn). The challenge is to find new
compounds that can control KPC producing-Kpn infections. The aim of this study was to evaluate the antibiotic activity of
the F3d fraction produced by the Pseudomonas aeruginosa LV strain against clinical isolates of KPC-producing Kpn. The
results showed that the minimum inhibitory concentration of F3d (62.5 µg mL-1), containing an organic metallic compound,
killed planktonic cells of KPC-producing Kpn strains after 30 min of incubation. At the same concentration, this
fraction also showed an inhibitory effect against biofilm of these bacteria after 24 h of incubation. Treatment with the F3d
fraction caused pronounced morphological alterations in both planktonic and biofilm cells of the bacteria. The inhibitory
effect of the F3d fraction seems to be more selective for the bacteria than the host cells, indicating its potential in the development
of new drugs for the treatment of infections caused by KPC-producing Kpn and other MDRO.
