Title:In vitro Evaluation of Gadolinium-Silica Mesoporous Nanoparticles- Monoclonal Antibody: Potential Nanoprobe for Prostate Cancer Cell Imaging
Volume: 4
Issue: 1
Author(s): Negar Ghalandarlaki, Noorahmad Latifi, Mehdi Mirzaei, Mohammad T. Joghatei, Sepideh Safari, Khadijeh Ashtari and Bita Mehravi
Affiliation:
Keywords:
Mesoporous silica nanosphere, MR imaging, monoclonal antibody C595, MUC-1, prostate cancer detection.
Abstract: Purpose: The unambiguous prostate cancer diagnosis is of high global interest. Magnetic
Resonance Imaging (MRI) is a low invasive modality in early stages of tumor diagnosis, especially for
cancer detection. The study aim is to synthesize Gd3+ based silica mesoporous nanosphere conjugated
with Monoclonal Antibody (Mab C595) and evaluate its ability as nanoprobe for prostate cancer cells
detection.
Method: Here, a nanoprobe, specifically recognizing in vivo MUC-1 antigen in prostate cancer cells, was synthesized and
evaluated. The in vitro studies included cell toxicity, cell binding and immune reactivity assay. MR imaging parameters of
this nanoprobe were investigated by MRI.
Results: Results showed that this nanoprobe is a selective MUC-1 detector, without any significant cell toxicity.
Conclusion: This study confirmed that this nanoprobe is potentially a selective prostate molecular imaging agent for cancer
cell detection.
