Title:Parametric Investigation of Batch Adsorption of Proteins onto Polymeric Particles
Volume: 16
Issue: 9
Author(s): Melvin X.L. Tan, Dominic Agyei, Sharadwata Pan and Michael K. Danquah
Affiliation:
Keywords:
Proteins, batch adsorption, liquid-solid mass transfer.
Abstract: Background: Effective bimolecular adsorption of proteins onto solid matrices is characterized
by in-depth understanding of the biophysical features essential to optimize the adsorption performance.
Results: The adsorption of bovine serum albumin (BSA) onto anion-exchange Q-sepharose
solid particulate support was investigated in batch adsorption experiments. Adsorption kinetics and
isotherms were developed as a function of key industrially relevant parameters such as polymer loading,
stirring speed, buffer pH, protein concentration and the state of protein dispersion (solid/aqueous) in order to optimize
binding performance and adsorption capacity. Experimental results showed that the first order rate constant is higher at
higher stirring speed, higher polymer loading, and under alkaline conditions, with a corresponding increase in equilibrium
adsorption capacity. Increasing the stirring speed and using aqueous dispersion protein system increased the adsorption
rate, but the maximum protein adsorption was unaffected. Regardless of the stirring speed, the adsorption capacity of the
polymer was 2.8 mg/ml. However, doubling the polymer loading increased the adsorption capacity to 9.4 mg/ml. Conclusions:
The result demonstrates that there exists a minimum amount of polymer loading required to achieve maximum protein
adsorption capacity under specific process conditions.
