Title:Curcumin Mitigates the Fibrillation of Human Serum Albumin and Diminishes the Formation of Reactive Oxygen Species
Volume: 22
Issue: 4
Author(s): Mansooreh Mazaheri, Ali Akbar Moosavi-Movahedi, Ali Akbar Saboury, Mehran Habibi Rezaei, Mostafa Shourian, Mohammad Farhadi and Nader Sheibani
Affiliation:
Keywords:
Aflatoxin M1, curcumin, fibrillation, HSA, lead ions, ROS.
Abstract: The formation of amyloid fibrils are thought to contribute to pathogenesis of many amyloids associated human
diseases. Here the impact of curcumin on amyloid formation of human serum albumin (HSA) was studied. Incubation of
HSA at 68°C under physiologic pH led to amyloid fibril formation. Thioflavin T (ThT) fluorescence was used for determination
of amyloid fibril formation. Atomic force microscopy experiments indicated different fibril structure of HSA incubated
with or without curcumin. The monitoring of the changes in reactive oxygen species (ROS) levels upon incubation
of curcumin with HSA showed a significant decrease in ROS levels. Similar experiments were also carried out in the
presence of aflatoxin M1 (AFM1) and lead (Pb) ions. Our results indicated that AFM1 and Pb ions promote the fibrillation
of HSA and accelerate ROS production, which were inhibited in the presence of curcumin. Thus, curcumin mitigates protein
fibrillation activity and diminishes ROS generation.
