Title:The Vasodilatation Effects of Four Active Components in Baige Capsule and the Potential Mechanism of Them with the NMDA Receptor
Volume: 11
Issue: 3
Author(s): Yuanji Wang, Yanmin Zhang, Weina Ma, Xiaoyan Pan, Nan Zhou, Bingling Dai and Langchong He
Affiliation:
Keywords:
Cellular membrane chromatography, MK-801, NMDA, vasodilation, imperatorin, puerarin.
Abstract: The vasodilatation effects of four components in Baige capsule were investigated on isolated
cerebral basilar arteries and mesenteric arteries model of rats. In order to inspect the potential
mechanism, the SH-SY5Y cellular membrane chromatography (CMC) was established to specially
recognize the ligand which could interact with N-methyl-D-aspartate receptor (NMDA). Frontal
chromatography and competitive binding chromatography were applied to investigate the interaction
of four main components in Baige capsule with this receptor. MK-801 (an antagonist of NMDA receptor)
was used as the displacer, ketamine hydrochloride, puerarin, daidzein, imperatorin, and isoimperatorin was used as
the ligand. The results of vasodilatation test suggested that imperatorin and isoimperatorin could effectively relax both of
the cerebral basilar arteries and mesenteric arteries. The CMC study showed that imperatorin and isoimperatorin could
competitively displace MK-801. The molecular docking model of the NMDA receptor indicated that imperatorin and isoimperatorin
showed a similar docking behavior as MK-801. Overall, the studies demonstrated that imperatorin and isoimperatorin
had obvious vasodilatation effect and might be prospective candidates as new NMDA receptor antagonists.
