Title:The Use of Citrullinated Peptides for the Diagnosis and Prognosis of Rheumatoid Arthritis
Volume: 14
Issue: 23
Author(s): Isabel Haro and Maria J. Gomara
Affiliation:
Keywords:
Rheumatoid Arthritis, Citrullinated peptides, Diagnosis, Prognosis.
Abstract: Rheumatoid arthritis (RA) is a chronic autoimmune disease that causes joint inflammation and
extra-articular manifestations. To prevent progressive and irreversible structural damage, early diagnosis of
RA is of paramount importance. Antibodies directed against citrullinated proteins and peptides (ACPAs)
are the most specific serological markers available for diagnosing RA. ACPAs may be detected several
years before symptoms appear, and their presence at disease onset is a good predictor of the development
of erosive joint lesions. Synthetic peptides can replace cognate proteins in solid-phase assays for specific autoantibody
recognition in RA patients. The use of synthetic peptides instead of proteins represents an advantage in terms of the reproducibility
of such immunoassays. They give absolute control over the exact epitopes presented. Furthermore, it is difficult
to prepare sufficient amounts of high-quality antigenic proteins with a well-defined degree of citrullination. Synthetic
citrullinated peptides, in contrast, are easily obtained in a pure form with a well-defined chemical structure and the epitopes
can be precisely oriented in the plate by covalent binding of the peptides. We have recently obtained and highlighted
the application of chimeric peptides bearing different citrullinated protein domains for the design of RA diagnosis systems.
Our results indicate that more than one serological test is required to classify RA patients based on the presence or
absence of ACPAs. Each of the target molecules reported (fibrin, vimentin and filaggrin) helps to identify a particular
subset of RA patients.
