Title:Antioxidant and Antifungal Potential of Pleurotus ostreatus and Agrocybe cylindracea Basidiocarps and Mycelia
Volume: 16
Issue: 2
Author(s): Jasmina Cilerdzic, Mirjana Stajic, Jelena Vukojevic, Ivan Milovanovic and Nikolina Muzgonja
Affiliation:
Keywords:
Agrocybe cylindracea, agro-industrial residues, antifungal activity, antioxidant activity, Pleurotus ostreatus;
submerged cultivation.
Abstract: Basidiocarps of Pleurotus ostreatus and Agrocybe cylindracea are characterized by high nutritional
value and numerous medicinal activities, though bioactivities of their mycelia have not been
sufficiently studied. The aim of the study was to evaluate antioxidant and antifungal potentials of
P. ostreatus and A. cylindracea basidiocarp extracts, as well as those obtained from mycelia cultivated in
common synthetic medium and media enriched with various agro-industrial residues. The free radical
scavenging activity of the extracts was determined spectrophotometrically, based on DPPH• reduction
while antifungal potential was studied by a microdilution method. The highest level of DPPH• scavenging ability was
obtained by an extract of P. ostreatus mycelium cultivated in wheat bran-enriched medium, while control medium
favoured the antioxidant potential of A. cylindracea mycelium. Phenol compounds were the main carriers of antioxidant
activity that was demonstrated by high coefficients for correlations between total phenol contents in extracts and level
of DPPH• scavenging (0.94 for P. ostreatus extracts and 0.91 for A. cylindracea extracts). Only the extracts of
A. cylindracea basidiocarp and control mycelium, at a concentration of 1.33 mg/mL, inhibited the growth of Microsporum
gypseum and Aspergillus flavus, while basidiocarp extract at 1.67 mg/mL also had fungicidal effect against M. gypseum.
These results revealed a considerable antioxidant potential of submergedly-cultivated mycelium which showed higher antioxidant
activity than basidiocarp extracts. This is very important because significant amounts of mycelium biomass
could be obtained more easily, cheaper and in a more controllable way than basidiocarps cultivation.
