Microwave-assisted Synthesis of HYNIC Protected Analogue for ^99mTc Labeled Antibody

Title:Microwave-assisted Synthesis of HYNIC Protected Analogue for ^99mTc Labeled Antibody

Volume: 7 Issue: 2

Author(s): Maria F. Garcia, Victoria Calzada, Ximena Camacho, Enzo Goicochea, Juan Pablo Gambini, Thomas P. Quinn, Williams Porcal and Pablo Cabral

Affiliation:

Keywords: 99m-technetium, HYNIC, microwave-assisted, nimotuzumab, trifluoroacetyl.

Abstract: We described herein a simple and efficient microwave assisted synthesis of HYNIC analogues. Two different activated esters of HYNIC, the hydrazine protected with a trifluoroacetyl group (5) and the free hydrazine (6) were conjugated to the monoclonal antibody Nimotuzumab. Technetium-99m radiolabeling of Nimotuzumab was achieved with high efficiency using 5 and 6 derivates. The NHS-HYNIC-Tfa derivate allowed better labeling yields during longer times of preservation of the conjugated antibody.

Cite this article as:

Garcia Maria F., Calzada Victoria, Camacho Ximena, Goicochea Enzo, Gambini Pablo Juan, Quinn P. Thomas, Porcal Williams and Cabral Pablo, Microwave-assisted Synthesis of HYNIC Protected Analogue for ^99mTc Labeled Antibody, Current Radiopharmaceuticals 2014; 7 (2) . https://dx.doi.org/10.2174/1874471007666141128160449