Title:Novel Pentablock Copolymer Based Nanoparticles Containing Pazopanib: A Potential Therapy for Ocular Neovascularization
Volume: 4
Issue: 1
Author(s): Varun Khurana, Sulabh P. Patel, Vibhuti Agrahari, Dhananjay Pal and Ashim K. Mitra
Affiliation:
Keywords:
Efflux transporters, ocular drug delivery, polymeric nanoparticles, sustained release, tyrosine kinase inhibitors.
Abstract: The main aim of this study was to design novel pentablock (PB) (PLA–PCL–PEG–PCL–PLA) polymer to
prepare nanoparticles (NP) in order to achieve sustain delivery of pazopanib with minimal burst effect for the treatment of
ocular neovascularization. Another purpose was to evaluate the effect of pazopanib loaded NP to bypass drug efflux with
the discussion of recent patents. PB copolymer was successfully synthesized using ring opening polymerization reaction
mechanism and characterized using 1H NMR, GPC and XRD analysis. Synthesized PB copolymer was found to noncytotoxic,
non-immunogenic and biocompatible with ocular cell lines. Also, several parameters such as entrapment
efficiency, drug loading, in vitro drug release profiling and effect of pazopanib NP in evading efflux transporters were
examined. PB copolymer-based NP exhibited continuous release of pazopanib. It can be utilized to achieve continuous
first order delivery of pazopanib upto 100 days from nanoparticles without any significant burst effect. Pazopanib loaded
NP were successful in evading drug efflux mediated via efflux transporters. This formulation can be employed to
circumvent ocular barriers without altering ocular protective mechanisms. Our results indicated that PB copolymer based
drug delivery systems can serve as a platform technology for the development of sustained release therapy for the
treatment of ocular neovascularization. This drug delivery system can also be applicable for other ocular complications.
