Title:Development and Characterization of Amphotericin B Nanosuspensions for Oral Administration Through a Simple Top-Down Method
Volume: 15
Issue: 6
Author(s): Zhiwen Yang, Min Liu, Jian Chen, Weijun Fang, Yanli Zhang, Man Yuan and Jie Gao
Affiliation:
Keywords:
AmB nanosuspensions, dissolution rate, magnetic stirring, oral, top-down.
Abstract: Amphotericin B (AmB) is regarded as a life-saving drug in treating severe systemic fungal infections. However,
the poor solubility and permeability limits its oral administration. The main purpose of this study is to evaluate
AmB nanosuspensions in enhancing its solubility for the oral application. Magnetic stirring method with very low energy
input, as a top-down technology, was firstly used to prepare the drug nanosuspensions. Sodium deoxycholate and carbomer
were screened as the stabilizers through a single-factor experiment. Under the optimum conditions, AmB nanosuspensions
were spherically shaped with the average particle size of 348.9±21.2 nm. X-ray diffraction analysis and differential
scanning calorimetry confirmed that the initial crystalline state was preserved after particle size reduction. Saturated
solubility and dissolution rate of AmB nanosuspensions exhibited the better dissolution properties compared with raw
drug. Stability study demonstrated that AmB nanosuspensions maintained a good stability at 4oC for 30 days. In conclusion,
AmB nanosuspensions potentially improved the oral absorption and magnetic stirring method could be acted as an
effective method to produce AmB nanosuspensions.
