Title:Advances in Nanomaterial-Based Microwaves and Infrared Wave-Assisted Tryptic Digestion for Ultrafast Proteolysis and Rapid Detection by MALDI-MS
Volume: 17
Issue: 1
Author(s): Suresh Kumar Kailasa and Hui-Fen Wu
Affiliation:
Keywords:
Infrared waves, MALDI-MS, microwaves, nanomaterials, proteolysis.
Abstract: The unique physical/chemical properties of nanomaterials have significant impacts in electromagnetic waves
(microwave and infrared waves)-assisted tryptic digestion approaches by using them as heat absorbers to expedite
digestion and as affinity probes to enrich digested proteins prior to MALDI-MS analysis. We review recent developments
in electromagnetic waves (microwaves and infrared waves)-assisted proteolysis using nanomaterials as heat absorbers and
as affinity probes for analysis of digested proteins in MALDI-MS. New trends in ultrafast proteolysis
(nonphosphoproteins- lysozyme, cytochrome c, myoglobin and bovine serum albumin (BSA); phosphoproteins- α- and β-
caseins) using nanomaterials based microwaves and infrared (IR) waves assisted digestion approaches for rapid
identification of digested proteins in the MALDI-MS.
