Title:Generation of Human Cardiomyocytes for Cardiac Regenerative Therapies: Differentiation and Direct Reprogramming
Volume: 20
Issue: 12
Author(s): Chani Hodonsky, Kaihong Wu, Lakshmi Mundada and Ming-Sing Si
Affiliation:
Keywords:
Cardiomyocyte, cardiac differentiation, direct reprogramming, pluripotent stem cells, microRNA, forward programming.
Abstract: The generation of functional human cardiomyocytes carries the potential of replacing damaged, malformed, or congenitally
absent cardiac tissue as a definitive cure for cardiac disease. Furthermore, patient-specific cardiomyocytes may yield useful in vitro models
of heart tissue for disease investigation, drug development and personalized therapy evaluation. This field has experienced rapid advances
in the past few years. Nearly pure populations of cardiomyocytes have been generated from human pluripotent stem cells and new
strategies to generate cardiomyocytes from somatic cells have been introduced. Here we review the latest breakthroughs in cardiomyocyte
differentiation from human pluripotent stem cells and the creation of cardiomyocytes by direct reprogramming strategies, as well as
discuss their limitations.
