Title:Electrochemical Determination of Glutathione in Plasma at Carbon Nanotubes Based Screen Printed Electrodes
Volume: 16
Issue: 9
Author(s): Ezgi Turunc, Hakan Karadeniz, Guliz Armagan, Arzum Erdem and Ayfer Yalcin
Affiliation:
Keywords:
Differential pulse voltammetry, glutathione, multi-walled carbon nanotubes, plasma samples, screen-printed
electrodes, spectrophotometry.
Abstract: Glutathione (GSH) is a major endogenous antioxidant highly active in human tissues and plays a key role in
controlling cellular thiol redox system, maintaining the immune and detoxification system. The determination of GSH
levels in tissue is important to estimate endogenous defenses against oxidative stress. In our study, the multi-walled
carbon nanotube modified screen-printed electrodes (MWCNT-SPEs) were used to determine the levels of GSH in
trichloroacetic acid (TCA)-treated or untreated samples of rat plasma. It was found that the deproteinization of samples
with TCA improved the electrochemical detection of GSH particularly in plasma. The oxidation of GSH was measured by
using differential pulse voltammetry (DPV) method in combination with MWCNT-SPE (n=3), and the detection limit of
GSH was found to be 0.47 µM (S/N=3). The GSH levels in plasma samples were also measured spectrophotometrically in
order to compare the effectiveness of electrochemical method and we obtained a high correlation between the two
methods (R2=0.976).
