Title:A Mini Review of qRT-rtPCR Technology Application in Uncovering the Mechanism of Food Allergy and in the Search for Novel Interventions
Volume: 12
Issue: 1
Author(s): Elie K. Barbour, Houssam A. Shaib, Diana M. Ahmadieh, Taha Kumosani, Shady K. Hamadeh, Esam Azhar and Steve Harakeh
Affiliation:
Keywords:
Experimental designs, food allergy, interventions, mechanism, novel, qRT-rtPCR
Abstract: This mini review targets the inclusion of recent selected citations, between the year 2006 and 2012, that
implement the qRT-rtPCR technology in their experimental designs, targeting the uncovering of the mechanism of food
allergy. In addition, this same technology was implemented in specific experimental designs, aiming at finding novel
nutritional, herbal medicine, and tolerance interventions against food allergy. The approach of using qRT-rtPCR
technology helped in studying the dynamics of transcription of cytokines and chemokines in intestinal dendritic cells of
the experimental animals during the allergic reaction to food. The suppression of transcription of specific cytokines or
chemokines by nutritional, herbal medicine, and tolerance interventions was instrumental in the search for finding novel
remedies for this health condition, that was traditionally managed by avoidance of offending foods in the diet.
