Title:Identification of a Novel Peptide Ligand of Human Transfrrin Receptor 1 for Targeted Tumor Delivery Drug
Volume: 20
Issue: 1
Author(s): Qin Xin, Zhang Cun, Xue Xiaochang, Li Meng, Li Weina, Hao Qiang, Zhang Yingqi, Yan Zhen and Zhang Wei
Affiliation:
Keywords:
TfR1, phage display, peptide, malignant cells, normal cells, tumor, cancer progression, flow cytometry assay
Abstract: Human transferrin receptor 1 (TfR1) is expressed on malignant cells at levels several fold higher than those on
normal cells and its expression can be correlated with tumor stage or cancer progression. It is a potentially rational target
for drug delivery. To identify novel ligands which can recognize the TfR1 specifically, a random phage displayed 12-mer
peptide library was screened and two consensus motifs of the peptides which are displayed by the positive phages RXR
and RXXXR (x is any amino acid) were yield. The phage displaying peptide SPRPRHTLRLSL (designated as B18) exhibited
the highest affinity to TfR1 in phage ELISA and B18 could bind to TfR1 specifically in a dose dependent manner.
The flow cytometry assay and immunocellularchemistry assay showed that the B18 could bind to TfR1 positive carcinoma
cells with specificity. In addition, the biodistribution assay indicated that B18 could home to TfR1 positive tumor
tissue specifically. Our study suggests that the peptide exhibited by B18 is a potentially promising targeting peptide for
tumor diagnosis and treatment.
