Title:In Vitro Infection of Human Umbilical Cord Blood CD34+ Hematopoietic Progenitor Cells by HIV-1 CRF07_BC Enveloped Pseudovirus
Volume: 10
Issue: 7
Author(s): Lin Li, Chao Qiu, Liangzhu Li, Aiping Liu, Mingzhe Zhou, Zhimin Han, Chenli Qiu, Xiaoyan Zhang, Jianqing Xu and Huanzhang Zhu
Affiliation:
Keywords:
HIV-1, latency, hematopoietic progenitor cells, CRF07_BC, integration
Abstract: To determine whether CRF07_BC, one of the most predominant strains that accounts for one third HIV-1
prevalence in China, has the ability to infect hematopoietic progenitor cells (HPCs), human Umbilical Cord Blood (UCB)
derived CD34+ HPCs isolated with high purity were infected by HIV-1 pseudotyped with CRF07_BC envelope. After
HIV-1 infection, ~0.86% CD34+ HPCs were co-stained for CD34 and intracellular HIV Gag. HIV p24 antigen was
detectable and reached maximal release between day 2-4 after HIV-1 infection. The data of nested Alu-LTR PCR proved
the integration of HIV-1 genome into the host genome occurred in HIV-1-infected HPCs. These data demonstrated that
the envelope of CRF07_BC from China has the capability of resulting in infection to CD34+ HPCs, which may serve as a
mechanism for long-term latency of HIV-1 infection in vivo.
