Title:Optimized and Comparative Antioxidant Assays and Its Applications in Herbal and Synthetic Drug Analysis as an Antioxidants
Volume: 12
Issue: 10
Author(s): Shivraj Hariram Nile, C. N. Khobragade and Se Won Park
Affiliation:
Keywords:
Herbal drugs, Synthetic drugs, Antioxidant, Assays.
Abstract: Drug development in the recent times often relies on use of natural and synthetic drugs that are promising
candidates as therapeutic agents for prevention of diseases and disorders. They possess different chemical structures with
wide range of therapeutic activities. Many natural and synthetic drugs act as antioxidant agents in various metabolic
processes. Increasing epidemiological, clinical and experimental studies have shown that intake of antioxidants drugs
provide protection against various disorders and diseases related to oxidative stress. The factors responsible for this
oxidative stress are mainly free radicals, reactive nitrogen species (RNS) and reactive oxygen species (ROS). The
antioxidant drugs act as free radical scavenging, reducing and metal chelating substances; Antioxidants also show
inhibition of various metabolic enzymes and factors responsible for inflammation. The present paper reviews different In
vitro assays for determination of antioxidant activities (Table 1). The basic assays include DDPH assay, OH Scavenging
assay, Reducing activity assay, TEAC assay, FCR assay, PRTC assay, ABTS assay, FRAP assay, ORAC assay, Ferric
thiocynate assay, TRAP assay, Chemiluminescence assay, NBT assay, CUPRAC Assay.
