Title: Oxidation Proteomics: The Role of Thiol Modifications
Volume: 6
Issue: 1
Author(s): Beat M. Riederer
Affiliation:
Keywords:
Aging, cysteine, oxidation, thiol, DIGE, MALDI-TOF, Western blot, dye labeling, disease
Abstract: Identification of thiol modifications has gained significant importance. It is increasingly recognized that cysteines play an important role in protein function under both physiological and patho-physiological conditions. Here we reviewed different approaches that are used to identify oxidized proteins and discuss different fluorescent labeling techniques, differential two-dimensional gel electrophoresis and matrix-assisted laser desorption ionization – time of flight identification, in short MALDI-TOF. We illuminate processes that depend on protein oxidation of cysteines and we look
