Crystallization and Preliminary X-Ray Studies of the Fab Fragment from a Humanized Version of the Mouse Anti-Human Fas Antibody Hfe7a

Title: Crystallization and Preliminary X-Ray Studies of the Fab Fragment from a Humanized Version of the Mouse Anti-Human Fas Antibody Hfe7a

Volume: 9 Issue: 3

Author(s): Shuichiro Ito, Tomoko Takayama, Hiroyuki Hanzawa, Tohru Takahashi, Kenji Miyadai, Nobufusa Serizawa, Hideyuki Haruyama and Tadashi Hata

Affiliation:

Keywords: fab fragment, humanized hfe7a, fas monoclonal antibody

Abstract: A humanized version of the apoptosis-inducing mouse anti-human Fas monoclonal antibody, HFE7A, is under further development for the treatment of autoimmune diseases such as rheumatoid arthritis. We have crystallized the antigen-binding fragment (Fab) of the humanized HFE7A. The crystals belong to the orthorhombic space group P2 1 2 1 2 1 with cell dimensions a = 54.4 Å, b = 82.7 Å, c = 104.9 Å and contain one Fab molecule in the asymmetric unit. X-ray diffraction data were collected to 2.8 Å resolution.

Cite this article as:

Ito Shuichiro, Takayama Tomoko, Hanzawa Hiroyuki, Takahashi Tohru, Miyadai Kenji, Serizawa Nobufusa, Haruyama Hideyuki and Hata Tadashi, Crystallization and Preliminary X-Ray Studies of the Fab Fragment from a Humanized Version of the Mouse Anti-Human Fas Antibody Hfe7a, Protein & Peptide Letters 2002; 9 (3) . https://dx.doi.org/10.2174/0929866023408715