Expression Of Recombinant Xvax2 Peptide-104 Of Xenopus Laevis And Preparation Of The Antibody

Title: Expression Of Recombinant Xvax2 Peptide-104 Of Xenopus Laevis And Preparation Of The Antibody

Volume: 10 Issue: 2

Author(s): Yang Liu, Ying Liu, Wei Liu, Giuseppe Lupo, Rong-qiao He and Giuseppina Barsacchi

Affiliation:

Keywords: Recombinant, Xvax2 Peptide, Xenopus Laevis, N-terminal polypeptide, anti-XVAX2

Abstract: An N-terminal polypeptide (XVAX2-P104) of the XVAX2 protein, which is involved in controlling the dorsoventral patterning of the retina in Xenopus laevis, was expressed and purified as a Histagged fusion protein (pHis-XVAX2-P104), and it was employed to generate an anti-XVAX2 antibody in New Zealand white rabbits. ELISA analysis shows that the titer of the antibody is as high as ∼1:100,000. The antibody could specifically recognize the full-length XVAX2 protein in extracts of Xenopus embryos, as determined by Western Blotting.

Cite this article as:

Liu Yang, Liu Ying, Liu Wei, Lupo Giuseppe, He Rong-qiao and Barsacchi Giuseppina, Expression Of Recombinant Xvax2 Peptide-104 Of Xenopus Laevis And Preparation Of The Antibody, Protein & Peptide Letters 2003; 10 (2) . https://dx.doi.org/10.2174/0929866033479086