Isolation And Some Characterizations Of A Glycosylated Fibrinolytic Enzyme Of Earthworm, Eisenia Fetida

Title: Isolation And Some Characterizations Of A Glycosylated Fibrinolytic Enzyme Of Earthworm, Eisenia Fetida

Volume: 10 Issue: 2

Author(s): Li Li, Jing Zhao and Rong-Qiao He

Affiliation:

Keywords: earthworm fibrinolytic enzyme, glycosylated earthworm fibrinolytic enzyme, isolation, chromozym-th, affinity chromatography

Abstract: Resin coupled with m-aminophenylbornic acid was used to isolate a glycosylated component from homogenate of earthworm (Eisenia fetida). The fraction showed a single band on SDS-PAGE with a molecular weight of 34,193 Da determined by mass spectroscopy. The N-terminal region is AQVCCPDI, different from those of earthworm fibrinolytic enzymes reported previously (Nakajima et al. 1993). This glycosylated component showed an activity on digesting both Chromozym-TH and fibrin, suggesting that it is a novel fibrinolytic enzyme.

Cite this article as:

Li Li, Zhao Jing and He Rong-Qiao, Isolation And Some Characterizations Of A Glycosylated Fibrinolytic Enzyme Of Earthworm, Eisenia Fetida, Protein & Peptide Letters 2003; 10 (2) . https://dx.doi.org/10.2174/0929866033479095