An Efficient Fusion Expression System For Protein And Peptide Overexpression In Escherichia Coli And Nmr Sample Preparation

Title: An Efficient Fusion Expression System For Protein And Peptide Overexpression In Escherichia Coli And Nmr Sample Preparation

Volume: 10 Issue: 2

Author(s): Yuan Cheng, Dongsheng Liu, Yanming Feng and Guozhong Jing

Affiliation:

Keywords: protein expression, small fusion partner, nmr sample preparation

Abstract: An efficient fusion expression system with a small fusion partner, His6-tagged N-terminal fragment of staphylococcal nuclease R, has been constructed and tested with two genes. The results show that the system is not only suitable for overexpression of small proteins and peptides but simplifies purification of target proteins and peptides. The study also provides a practical method for preparation of isotope-labeled protein sample for NMR analysis.

Cite this article as:

Cheng Yuan, Liu Dongsheng, Feng Yanming and Jing Guozhong, An Efficient Fusion Expression System For Protein And Peptide Overexpression In Escherichia Coli And Nmr Sample Preparation, Protein & Peptide Letters 2003; 10 (2) . https://dx.doi.org/10.2174/0929866033479112