Synthesis of Chemotactic Peptide Analogs with a Photoaffinity Cross-Linker as New Probes for Analysis of Receptor-Ligand Interaction

Title: Synthesis of Chemotactic Peptide Analogs with a Photoaffinity Cross-Linker as New Probes for Analysis of Receptor-Ligand Interaction

Volume: 10 Issue: 2

Author(s): Mitsukuni Shibue, Masahiro Yoshiki, Masaya Miyazaki, Ichiro Fujita, Satoshi Osada, Seiji Yasuda, Hiroyuki Nakamura, Yuhei Hamasaki, Michio Kondo and Hideaki Maeda

Affiliation:

Keywords: chemotactic peptide, fmlp, formylpeptide receptor, photoaffinity labeling, cross-linking, p-benzoylphenylalanine, affinity tag, biotin

Abstract: Formylpeptide receptors are well-characterized receptors which participate in host defense responses of neutrophils. We designed and synthesized chemotactic peptide analog with pbenzoylphenylalanine (Bpa) and biotin to probe structural and mechanistic aspects of peptide-receptor interaction. These peptides possess biological activities which were dependent upon spacer residue length of and Bpa position. The covalent photoaffinity label was detected by Streptavidine-blot, which was inhibited by the parent peptide.

Cite this article as:

Shibue Mitsukuni, Yoshiki Masahiro, Miyazaki Masaya, Fujita Ichiro, Osada Satoshi, Yasuda Seiji, Nakamura Hiroyuki, Hamasaki Yuhei, Kondo Michio and Maeda Hideaki, Synthesis of Chemotactic Peptide Analogs with a Photoaffinity Cross-Linker as New Probes for Analysis of Receptor-Ligand Interaction, Protein & Peptide Letters 2003; 10 (2) . https://dx.doi.org/10.2174/0929866033479031