Title: Cardiac Oxidative Stress and Inflammation are Similar in SAMP8 and SAMR1 Mice and Unaltered by Curcumin and Ginkgo biloba Extract Intake
Volume: 11
Issue: 8
Author(s): Christina Schiborr, Gunter P. Eckert, Jakob Weissenberger, Walter E. Muller, Dorothea Schwamm, Tilman Grune, Gerald Rimbach and Jan Frank
Affiliation:
Keywords:
Aging, antioxidants, curcumin, Ginkgo biloba extract, heart health, inflammation, oxidative stress, senescenceaccelerated mice, cytokines, senescence-accelerated mouse-prone 8 (SAMP8), senescence-accelerated mouse-resistant 1 (SAMR1), protein carbonyls, glutathione, glutathione disulfide, methionine, cysteine, uric acid, tumor-necrosis factor-a, interleukin-1b, interleukin-6, Chronic inflammation, coronary heart disease (CHD), Curcuma longa, terpenelactones, flavonols quercetin, isorhamnetin, kaempferol, phytomedicine, Tissue Homogenates, phosphate-buffered saline, Protein Carbonyls, sulfuric acid, hemocyanin, streptavidin-biotinylated, Enzyme-Linked Immunosorbent Assay (ELISA), Glutathione disulfide, metaphosphoric acid, acetonitrile, isocratic elution, orthophosphoric acid, hydrophilic polypropylene, pathogenesis, atherosclerosis, Thiobarbituric acid reactive substances (TBARS)
Abstract: Chronic inflammation and oxidative stress increase with advancing age and appear to be involved in the pathogenesis of coronary heart disease, the leading cause of death worldwide. There is a need for animal models that reflect the increases in pro-inflammatory cytokines and oxidative damage observed during aging in humans. We therefore aimed to investigate the suitability of the fast-aging senescence-accelerated mouse-prone 8 (SAMP8) strain and its normally aging control senescence-accelerated mouse-resistant 1 (SAMR1) to study the age-dependent changes in cytokines, oxidative damage and antioxidants in the heart. To this end, 2-months-old male SAMR1 and SAMP8 mice were fed a Western type diet (control groups) for 5 months. Two groups of SAMP8 mice were simultaneously fed identical diets fortified with 0.5 g curcumin or 1.0 g Ginkgo biloba extract EGb 761® per kg diet. Heart tissue homogenates were analysed for protein carbonyls, glutathione, glutathione disulfide, methionine, cysteine and uric acid as well as the cytokines tumor-necrosis factor- α, interleukin-1β, interleukin-6, and monocyte chemoattractant protein-1. Neither the strain (SAMR1 or SAMP8) nor antioxidant intake (curcumin or EGb 761®) affected the concentrations of the measured parameters. In conclusion, our data do not support the suitability of the SAMP8 and SAMR1 strains as a model to study age-related changes in proinflammatory cytokines and oxidative stress parameters in the heart.
