Title: Real Time Analysis of Neurotransmitters in the Brain Using a Micro-Electrode System
Volume: 7
Issue: 1
Author(s): Suw Young Ly, Hwa Jin Heo and Min Jung Kim
Affiliation:
Keywords:
Brain, catechol, dopamine, epinephrine, live fish, micro carbon fiber, skeleton, voltammetry
Abstract: Neurotransmitters catechol and dopamine analogy levels are related to the systematic nerve activity. This assay was performed by using cyclic voltammetry (CV) and square wave (SW) stripping voltammetry, while optimization was performed by the carbon fiber microelectrode (CFME) with wire-type reference and counter electrodes. In the electrolyte solutions, the analytical parameters of catechol were as follows: 8.5 pH, -0.4 V anodic, 0.3 V cathodic, 15s deposition time, 15 Hz SW frequency, 25 mV SW amplitude and incremental potential of 4 mV, under optimal conditions, working ranges were 0-50 mgL-1 CV, 0.01-50 mgL-1 cathodic, and 1-13 mgL-1 anodic, the relative standard deviation was 0.02 %, n = 15 using 0.5 mgL-1 and the detection limit was 4.0 ugL-1. It can be used for in vivo direct analysis. Here, diagnostic applications were performed in the brain skeleton of living cells in real time. The developed techniques can be useful for nerve control and signal detections.
