Title:Paracetamol-induced Liver Injury in An Experimental Rat Model: Noninvasive
Assessment using DKI and the Effect of Gadoxetate on DKI Parameters
Volume: 20
Author(s): Lixue Xu, Dawei Yang, Dan Chen, Hui Xu, Jing Zheng, Tianxin Cheng, Hao Ren, Yan Wang, Xinyan Zhao*Zhenghan Yang*
Affiliation:
- Department of Liver Research Center, Beijing Friendship Hospital, Capital Medical University, Yongan Road 95, West District, Beijing, 100050,
China
- Department of Radiology, Beijing Friendship Hospital, Capital Medical University, Yongan Road 95, West District, Beijing, 100050, China
Keywords:
Drug-induced liver injury, Paracetamol, Diffusion kurtosis imaging, Gadoxetate, Rat model, ROC.
Abstract:
Purpose:
To explore the potential of diffusion kurtosis imaging (DKI) for assessing the degree of liver injury in a paracetamol-induced rat model and to
simultaneously investigate the effect of intravenous gadoxetate on DKI parameters.
Methods:
Paracetamol was used to induce hepatoxicity in 39 rats. The rats were pathologically classified into 3 groups: normal (n=11), mild necrosis (n=18),
and moderate necrosis (n=10). DKI was performed before and, 15 min, 25 min, and 45 min after gadoxetate administration. Repeated-measures
ANOVA with Tukey’s multiple comparison test was used to investigate the effect of gadoxetate on mean diffusivity (MD) and mean diffusion
kurtosis (MK) and to assess the differences in MD and MK among the three groups. A receiver operating characteristic (ROC) curve analysis was
performed to evaluate the diagnostic accuracy of the MD values when discriminating between the necrotic groups.
Results:
Gadoxetate had no significant effect on either the MD or the MK, and the effect size was small. The MD in the moderate necrosis group was
significantly lower than that in the other two groups (F = 13.502, p <0.001; η2 = 0.428 [95% CI: 0.082-0.637]), while the MK did not significantly
differ among the three groups (F = 2.702, p = 0.081; η2 = 0.131 [95% CI: 0.001-0.4003]). The AUCs of MD for discriminating the moderate
necrosis or normal group from the other groups were 0.921 (95% CI: 0.832-1.000) and 0.831 (95% CI: 0.701-0.961), respectively.
Conclusion:
It would be better to measure the MD and MK before gadoxetate injection. MD showed potential for assessing the degree of liver necrosis in a
paracetamol-induced liver injury rat model.
