Title:Anti-Inflammatory and Wound Healing Potential of Comphora Wightii,Herbal Ointment on Wistar Rats
Volume: 20
Issue: 2
Author(s): Ishu Garg*, Neelam Singh, Ishika Sharma, Jayeeta Dhingra and Kartik Kumar
Affiliation:
- I.T.S College of Pharmacy, Murad Nagar, Ghaziabad, Uttar Pradesh, India
Keywords:
Ointment, anti-inflammatory, wound, guggulu, sesame oil, ratanjot.
Abstract:
Background: A herbal approach to Guggulu (Commiphora wightii), as an anti-inflammatory
and wound healing agent is anticipated. Phyto-constituents Guggulusterone, Naringenin, and
myrrhanol were reported for the anti-inflammatory activity of Guggulu. Studies suggests, that sesame
oil (Sesamum indicum L.) and Ratanjot (Arnebia nobilis) also act as potent anti-inflammatory agents..
A combination of all three of these gives a synergistic effect for anti-inflammatory and excisional
wound healing activity.
Materials and Methods: A simple ointment base, BP and 5% Ratanjot, and Sesame oil were prepared.
All the components, i.e., Guggulu, 5% Ratanjot Sesame oil, and simple ointment base, were added
and triturated uni-directionally until a smooth, homogenous mixture was obtained. 25% w/w (F1) and
27% w/w (F2) Guggulu ointment were prepared. Four groups, each with three wistar rats, were studied
for fourteen days. On the fourteenth day, rats were sacrificed, and tissues were collected for histopathological
studies.
Results: F1 was compared against the standard formulation (10% w/w betadine, Win-Medicare) for
excisional wound healing and anti-inflammatory activity in rats. On the foutheenth day, the results
for percentage wound contraction in groups 1 (Negative control, vaseline), 2 (Controlled, ointment
base), 3 (F1), and 4 (positive control, standard) were found to be 12.01, 25.32, 96.14, and 81.23,
respectively. Results of histopathological studies and H&E staining supported the action of F1, as
skin sections showed the junction of normal skin and wound area. Sub-epithelial tissues showed low
(-) to mild (+) inflammation. Mild oedema (+) was also noted.
Conclusion: The rat group treated with F1 showed maximum wound contraction, healing, and antiinflammatory
activity as per percentage wound contraction and histopathological studies.
