Title:Single-cell Technology in Stem Cell Research
Volume: 20
Issue: 1
Author(s): Ali Golchin*, Forough Shams, Faezeh Moradi, Amin Ebrahimi Sadrabadi, Shima Parviz, Shahriar Alipour, Parviz Ranjbarvan, Yaser Hemmati, Maryam Rahnama, Yousef Rasmi and Shiva Gholizadeh-Ghaleh Aziz*
Affiliation:
- Cellular and Molecular Research Center, Cellular and Molecular Medicine Institute, Urmia University of Medical
Sciences, Urmia, Iran
- Department of Clinical Biochemistry and Applied Cell Sciences, School of Medicine, Urmia
University of Medical Sciences, Urmia, Iran
- Department of Clinical Biochemistry and Applied Cell Sciences, School of Medicine, Urmia
University of Medical Sciences, Urmia, Iran
Keywords:
Single-cell technology, stem cell, sc-RNA-seq, smart-seq, drop-seq, tissue engineering.
Abstract: Single-cell technology (SCT), which enables the examination of the fundamental units
comprising biological organs, tissues, and cells, has emerged as a powerful tool, particularly in
the field of biology, with a profound impact on stem cell research. This innovative technology
opens new pathways for acquiring cell-specific data and gaining insights into the molecular pathways
governing organ function and biology. SCT is not only frequently used to explore rare and
diverse cell types, including stem cells, but it also unveils the intricacies of cellular diversity and
dynamics. This perspective, crucial for advancing stem cell research, facilitates non-invasive analyses
of molecular dynamics and cellular functions over time. Despite numerous investigations into
potential stem cell therapies for genetic disorders, degenerative conditions, and severe injuries,
the number of approved stem cell-based treatments remains limited. This limitation is attributed to
the various heterogeneities present among stem cell sources, hindering their widespread clinical
utilization. Furthermore, stem cell research is intimately connected with cutting-edge technologies,
such as microfluidic organoids, CRISPR technology, and cell/tissue engineering. Each strategy
developed to overcome the constraints of stem cell research has the potential to significantly
impact advanced stem cell therapies. Drawing on the advantages and progress achieved through
SCT-based approaches, this study aims to provide an overview of the advancements and concepts
associated with the utilization of SCT in stem cell research and its related fields.
