Title:Neuroprotective Effects of Isolated Mangiferin from Swertia chirayita Leaves Regulating Oxidative Pathway on Streptozotocin-Induced Diabetic
Neuropathy in Experimental Rats
Volume: 24
Issue: 2
Author(s): Shivam*Asheesh Kumar Gupta
Affiliation:
- Faculty of Pharmacy, School of Pharmaceutical Sciences, IFTM University Delhi Road, NH-24 Moradabad, Lodhipur
Rajput, Uttar Pradesh 244102, India
Keywords:
Diabetic neuropathy, mangiferin, diabetes, Swertia chirayita, oxidative stress, inflammation, inflammatory cytokines.
Abstract:
Background: Oxidative stress has an important role in the pathogenesis and development
of diabetic peripheral neuropathy (DPN), the most common and debilitating complication of
diabetes mellitus. Swertia chirayita is a rich source of phenolic constituents and has hypoglycemic,
anti-inflammatory, and antioxidant properties.
Aims: This study was performed to evaluate the neuroprotective effect in diabetes by enhancing
antioxidant defense against oxidative stress, which exhibits a neuroprotective effect in streptozotocin-
induced diabetic rats.
Objectives: The objective of this study was to elucidate the therapeutic potential of bioactive
compounds of Swertia chirayita for diabetic complications.
Methods: The present work focused on isolating the bioactive from the leaves of Swertia absinthe
for acute toxicity studies, assessing its protective effects against diabetes and diabetic neuropathy
as well as its mode of action in STZ-induced Wistar rats. The local area of Moradabad is the place
from where the leaves of Swertia chirayita were gathered. Mangiferin was isolated and identified
using spectroscopic techniques, such as UV, HPLC, 1H NMR, C13 NMR, MAS, and FTIR.
Mangiferin was administered in doses of 15 and 30 mg/kg to test its effect on experimentally
induced diabetes. The sciatic nerves of all groups were examined histopathologically. The
protective effect of the drug against diabetes and diabetic neuropathy was demonstrated by
measures, such as blood glucose level, body weight, food intake, thermal hyperalgesia, grip
strength, spontaneous locomotor test, and lipid profile analysis. Sciatic nerve cells of the treated
groups showed less inflammation, degeneration, and necrosis.
Results: The results of this study confirmed that mangiferin alleviated diabetic neuropathic pain,
possibly by reducing inflammatory cytokines (TNF-α, TGF-β1, IL-1β, and IL-6), strong
antioxidant activity, and NGF in sciatic nerves. It may be a therapeutic agent.
Conclusion: Our results suggested that active phytochemicals of Swertia chirayita showed preventive
and curative effects against STZ-induced diabetic neuropathy in rats, which might be due to its
antioxidant, anti-inflammatory, and anti-apoptotic properties.
