Regulation of ROS/inflammasome Axis is Essential for Cardiac Regeneration in Aging Rats Receiving Transplantation of Mesenchymal Stem Cells

Wei-Syun      Hu; Jing-Yi      Chen; Wei-Yu      Liao; Chin-Hsien      Chang; Tung-Sheng      Chen

doi:10.2174/011574888X276612231121065203

Abstract

Background: Aging is a biological and gradual deterioration of function in living organisms. Aging is one of the risk factors for heart disease.

Objective: Although mesenchymal stem cell transplantation shows potential in heart disease treatment, the relationship between stem cell-based therapy and oxidative stress/inflammasome axis regulation remains unclear. This study hypothesized that intervention of stem cells showed protective effect on heart aging induced by D-galactose through regulation of oxidative stress/inflammasome axis.

Methods: An aging animal model was designed to test the above hypothesis. Experimental animals were divided into three groups, including Sham, D-gal (aging rats induced by d-galactose), and D-gal+WJSC (aging rats receiving mesenchymal stem cells).

Results: Compared to the Sham, the experimental results indicate that structural alteration (HE stain and Masson’s Trichrome stain), oxidative stress elevation (increase of TBARS level, expression of gp-91 and suppression of Sirt-1 as well as SOD2), increase of aging marker p53, suppression of cardiogenesis marker Troponin T, and inflammasome related protein markers expression (NLRP3, caspase-1 and IL-1 beta) were significantly observed in D-gal. In contrast, all pathological pathways were significantly improved in D-gal+WJSC when compared to D-gal. In addition, migration of stem cells to aging heart tissues was observed in the D-gal+WJSC group.

Conclusion: These findings suggest that mesenchymal stem cell transplantation effectively ameliorates aging hearts through oxidative stress/inflammasome axis regulation. The results from this study provide clinical potential for stem cell-based therapy in the treatment of aging hearts.

Keywords: Aging, cardiomyopathy, mesenchymal stem cells, inflammasome, oxidative stress, transplantation.

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DOI https://dx.doi.org/10.2174/011574888X276612231121065203	Print ISSN 1574-888X
Publisher Name Bentham Science Publisher	Online ISSN 2212-3946

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Regulation of ROS/inflammasome Axis is Essential for Cardiac Regeneration in Aging Rats Receiving Transplantation of Mesenchymal Stem Cells

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