Title:Naringenin-induced Oral Cancer Cell Apoptosis Via ROS-mediated Bid
and Bcl-xl Signaling Pathway
Volume: 24
Issue: 6
关键词:
柚皮素，口腔癌，ROS, Bid, Bcl-xl，信号通路，细胞凋亡。
摘要:
Background: Oral cancer is a malignant tumor with a high impact and poor prognosis.
Naringenin, a flavonoid found in citrus fruits and its anti-inflammatory and antioxidant properties
offer potential therapeutic benefits. However, limited studies have been conducted on the impact
of naringenin on human tongue carcinoma CAL-27 cells. This study aims to elucidate the correlation
between naringenin and tongue cancer, thereby identifying a potential therapeutic candidate
for drug intervention against tongue cancer.
Methods: The effect of naringenin on the apoptosis of CAL-27 cells and its mechanism were
studied by cell counting kit-8, mitochondrial membrane potential assay with JC-1, Annexin V--
FITC apoptosis detection, cell cycle, and apoptosis analysis, Reactive Oxygen Species assay and
Western blot.
Results: The results showed that naringenin significantly induced apoptosis in CAL-27 cells in a
dose-dependent manner. Mechanistically, naringenin-induced apoptosis was mediated through the
upregulation of Bid and downregulation of Bcl-xl, which led to increased generation of ROS.
Conclusion: The findings suggested that naringenin may represent a promising candidate for the
treatment of oral cancer by inducing apoptotic cell death via modulation of the Bid and Bcl-xl signaling
pathways.
